Detailed information NTG0871864

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RBI07_RS02095
• Genome accession: NZ_CP133094
• Coordinates: 448003..449151 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain pae001
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 443003..454151

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RBI07_RS02070 (RBI07_02070)	-	444154..444450 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
RBI07_RS02075 (RBI07_02075)	-	444447..445040 (-)	594	WP_003084544.1	YggT family protein	-
RBI07_RS02080 (RBI07_02080)	proC	445051..445872 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
RBI07_RS02085 (RBI07_02085)	-	445884..446576 (-)	693	WP_010793543.1	YggS family pyridoxal phosphate-dependent enzyme	-
RBI07_RS02090 (RBI07_02090)	pilT	446791..447825 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
RBI07_RS02095 (RBI07_02095)	pilU	448003..449151 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
RBI07_RS02100 (RBI07_02100)	czcD	449159..450058 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
RBI07_RS02105 (RBI07_02105)	-	450212..450616 (+)	405	WP_003084560.1	NINE protein	-
RBI07_RS02110 (RBI07_02110)	-	450859..452232 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
RBI07_RS02115 (RBI07_02115)	-	452229..453413 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=871864 RBI07_RS02095 WP_003100959.1 448003..449151(+) (pilU) [Pseudomonas aeruginosa strain pae001]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=871864 RBI07_RS02095 WP_003100959.1 448003..449151(+) (pilU) [Pseudomonas aeruginosa strain pae001]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382