Detailed information NTG0871830

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RBI06_RS02335
• Genome accession: NZ_CP133093
• Coordinates: 521479..522627 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain 002
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 516479..527627

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RBI06_RS02310 (RBI06_02310)	-	517630..517926 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
RBI06_RS02315 (RBI06_02315)	-	517923..518516 (-)	594	WP_003084544.1	YggT family protein	-
RBI06_RS02320 (RBI06_02320)	proC	518527..519348 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
RBI06_RS02325 (RBI06_02325)	-	519360..520052 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
RBI06_RS02330 (RBI06_02330)	pilT	520267..521301 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
RBI06_RS02335 (RBI06_02335)	pilU	521479..522627 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
RBI06_RS02340 (RBI06_02340)	czcD	522635..523534 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
RBI06_RS02345 (RBI06_02345)	-	523689..524093 (+)	405	WP_003084560.1	NINE protein	-
RBI06_RS02350 (RBI06_02350)	-	524336..525709 (+)	1374	WP_003459266.1	pyridoxal-phosphate dependent enzyme	-
RBI06_RS02355 (RBI06_02355)	-	525706..526890 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=871830 RBI06_RS02335 WP_003110016.1 521479..522627(+) (pilU) [Pseudomonas aeruginosa strain 002]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=871830 RBI06_RS02335 WP_003110016.1 521479..522627(+) (pilU) [Pseudomonas aeruginosa strain 002]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382