Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   RBI12_RS01725 Genome accession   NZ_CP133054
Coordinates   365376..366155 (+) Length   259 a.a.
NCBI ID   WP_017481731.1    Uniprot ID   A0A9X3QKA0
Organism   Acinetobacter pittii strain A774     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 360376..371155
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RBI12_RS01710 (RBI12_01715) ponA 360957..363518 (-) 2562 WP_017481733.1 penicillin-binding protein PBP1a -
  RBI12_RS01715 (RBI12_01720) pilM 363680..364738 (+) 1059 WP_005067078.1 pilus assembly protein PilM Machinery gene
  RBI12_RS01720 (RBI12_01725) pilN 364738..365379 (+) 642 WP_017481732.1 PilN domain-containing protein Machinery gene
  RBI12_RS01725 (RBI12_01730) pilO 365376..366155 (+) 780 WP_017481731.1 type 4a pilus biogenesis protein PilO Machinery gene
  RBI12_RS01730 (RBI12_01735) pilP 366167..366694 (+) 528 WP_017481730.1 pilus assembly protein PilP Machinery gene
  RBI12_RS01735 (RBI12_01740) pilQ 366757..368883 (+) 2127 WP_017481729.1 type IV pilus secretin PilQ Machinery gene
  RBI12_RS01740 (RBI12_01745) aroK 368895..369464 (+) 570 WP_002116987.1 shikimate kinase AroK -
  RBI12_RS01745 (RBI12_01750) aroB 369493..370575 (+) 1083 WP_017481728.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 259 a.a.        Molecular weight: 29463.60 Da        Isoelectric Point: 5.7424

>NTDB_id=871529 RBI12_RS01725 WP_017481731.1 365376..366155(+) (pilO) [Acinetobacter pittii strain A774]
MSPDELHELSLEQAPSKKKKITLEKFLQQFNTLDMNSYGSWPLSVKITCWIFIFFAVLVLGYFVAIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQLQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
ITIEATGDYHAFGAFVSSIAALPRIVTMHDFVVDAAPIKDGKSDIPVLNYSIKAKTYRYMGAVENQNNSDPQVINGESQI
NASDSRNVHIPSIKKSKRK

Nucleotide


Download         Length: 780 bp        

>NTDB_id=871529 RBI12_RS01725 WP_017481731.1 365376..366155(+) (pilO) [Acinetobacter pittii strain A774]
ATGAGTCCTGATGAACTTCATGAGCTGTCTTTAGAACAAGCTCCATCTAAGAAAAAAAAGATCACCTTAGAAAAATTTCT
TCAGCAATTTAACACGCTCGACATGAACAGTTATGGCAGCTGGCCATTATCTGTGAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTACTGGTTTTAGGGTATTTTGTTGCTATTCAACCAAAGCTACAAGCAATTGATAATGCTCAAGCACAAGAA
AGTAATTTATTAAATGAGTTTCGAGAAAAAGACTCAAAATTACGCAACTTGCAGCAATACCAGCTTCAGCTTCAAGAGAT
GCAAGCCAACTTCAATCAGCAGTTAGAACAATTGCCAAAAGAAACCGAAATTCCAAGTTTAGTTGAAGATATTAACTTAA
CGGGGGTGAATTCTGGTCTGAAGTTTAAAAATATCCGCTTAGAAGATGAAGTGAAGCAGGAAATTTTTATTGAACAGCCT
ATTACTATCGAAGCAACTGGGGATTATCACGCTTTCGGTGCTTTTGTTAGCAGTATTGCGGCATTACCACGTATTGTGAC
AATGCATGATTTTGTTGTTGACGCTGCTCCTATTAAGGATGGTAAATCAGATATTCCTGTGCTTAATTATTCTATTAAAG
CAAAAACTTATCGCTATATGGGTGCGGTTGAAAACCAAAATAATAGTGATCCTCAAGTGATAAATGGTGAAAGTCAGATA
AACGCCTCCGATAGCCGTAATGTACATATTCCTTCTATAAAGAAGTCAAAGCGAAAGTAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

89.732

86.486

0.776
  comO Acinetobacter baylyi ADP1

71.245

89.961

0.641