Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RBI20_RS16275 Genome accession   NZ_CP133046
Coordinates   3507507..3509240 (-) Length   577 a.a.
NCBI ID   WP_308679060.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain 11066     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3502507..3514240
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RBI20_RS16260 (RBI20_16260) sucC 3502806..3503975 (-) 1170 WP_004154430.1 ADP-forming succinate--CoA ligase subunit beta -
  RBI20_RS16265 (RBI20_16265) - 3504333..3505946 (+) 1614 WP_005410794.1 ATP-binding protein -
  RBI20_RS16270 (RBI20_16270) pilR 3506007..3507380 (+) 1374 WP_049452342.1 sigma-54 dependent transcriptional regulator Regulator
  RBI20_RS16275 (RBI20_16275) pilB 3507507..3509240 (-) 1734 WP_308679060.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RBI20_RS16280 (RBI20_16280) - 3509463..3509870 (-) 408 WP_308679062.1 pilin -
  RBI20_RS16285 (RBI20_16285) - 3510022..3510459 (-) 438 WP_308679063.1 pilin -
  RBI20_RS16290 (RBI20_16290) pilC 3510812..3512071 (+) 1260 WP_308679065.1 type II secretion system F family protein Machinery gene
  RBI20_RS16295 (RBI20_16295) - 3512079..3512942 (+) 864 WP_005410800.1 A24 family peptidase -
  RBI20_RS16300 (RBI20_16300) coaE 3512954..3513565 (+) 612 WP_308679067.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62400.31 Da        Isoelectric Point: 5.8722

>NTDB_id=871423 RBI20_RS16275 WP_308679060.1 3507507..3509240(-) (pilB) [Stenotrophomonas maltophilia strain 11066]
MNAVTTANLVGITGLARRLVQDGALDEATARDAMAKAAAARQPLPTWFAQNKLVNASQLAAANAVEFGMPLFDVSTFDAN
QNAMSLVSEELLRKHNVLPLFRRGGRLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTLGDALGGD
DDGMGNLDVAAGDEDLGATGGDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPV
KLNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKL
FLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCGNCKRPSSLPE
HALLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGVYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKK
AAAGITSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=871423 RBI20_RS16275 WP_308679060.1 3507507..3509240(-) (pilB) [Stenotrophomonas maltophilia strain 11066]
ATGAACGCTGTCACCACCGCCAATCTTGTCGGCATCACCGGCCTGGCCCGTCGCCTGGTGCAGGACGGGGCCCTGGACGA
GGCCACCGCCCGCGACGCCATGGCCAAGGCCGCGGCCGCCCGCCAGCCACTGCCGACGTGGTTCGCTCAGAACAAGCTGG
TCAATGCCTCGCAGCTGGCCGCCGCCAACGCGGTCGAGTTCGGCATGCCGCTGTTCGACGTGTCCACCTTTGACGCCAAC
CAGAACGCGATGAGCCTGGTCAGCGAGGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAGGCGCGGTGGCAGGCT
GTTCGTCGGCACCAGCAACCCGACCCACTCGCTGGACGAGATCAAGTTCCACACCAATCTGGTGGTCGAACCGATCCTGG
TTGACGAGGACCAGATCCGCCGCACGCTGGAACAGTGGCACGCCAGCCATGACACCCTCGGCGACGCCCTGGGGGGCGAT
GACGATGGCATGGGCAACCTGGACGTGGCGGCCGGCGACGAGGATCTCGGCGCCACCGGCGGCGATTCCGGCATCGACGC
CAAGGGTGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTCGATGCGATCCGCAAGGGTGCCTCGGACATCC
ACTTCGAGCCCTACGAAGATGATTACCGCGTGCGCCTGCGCATCGATGGCCTGCTGAAGATGGTCGCGCGCGCACCGGTG
AAGTTGAACCAGCGCATCGCCGCCCGCCTGAAGGTGATGGCACAGCTGGACATCGCCGAGAAGCGCGTCCCGCAGGACGG
CCGCATCAAGCTCAATCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACCCTGTTCGGCGAGAAGG
TGGTGCTGCGTATCCTCGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAGCAGAAGCTG
TTCCTGGATGCCATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTGTA
TACCGCGCTGGGCATCCTCAACGACGAGACGCGCAACATCTCCACCGCCGAAGACCCGGTGGAAATCCGCCTGCCGGGCG
TCAACCAGGTACAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGACAGGACCCGGAC
ATCATCATGGTCGGCGAAATCCGCGACCTGGAAACAGCGGAGATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGCT
GTCCACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGTATCGCGCCCTACAACATCACCA
GCTCGGTGACGCTGGTGATCGCCCAGCGCCTGGCGCGTCGCCTGTGCGGCAACTGCAAGCGACCGTCCAGCCTGCCCGAA
CATGCGCTGCTCGCCGAAGGCTTCACCCAGGCGCAGCTGGATGCGGGCATCGAGCTGTACGAGGCCGTGGGCTGCGACGA
ATGCACCGAGGGCTACAAGGGCCGGACCGGCGTCTACCAGGTCATGCCGATGACCGATGAGATCGCCACCATCGTGCTGG
CCGGTGGCAATGCGCTGCAGATTGCCGAGGCGGCGCAGCAGATCGGGGTGAATGACCTGCGCCAGTCGGCATTGAAGAAG
GCGGCGGCGGGCATTACCAGCTTGGCCGAGATCAACCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.872

100

0.556
  pilB Acinetobacter baumannii D1279779

54.828

100

0.551
  pilB Legionella pneumophila strain ERS1305867

51.93

98.787

0.513
  pilF Neisseria gonorrhoeae MS11

48.772

98.787

0.482
  pilB Vibrio cholerae strain A1552

48.501

98.267

0.477
  pilB Vibrio parahaemolyticus RIMD 2210633

45.804

99.133

0.454
  pilB Vibrio campbellii strain DS40M4

45.518

98.614

0.449