Detailed information NTG0871196

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RBH03_RS16505
• Genome accession: NZ_CP132995
• Coordinates: 3539824..3540972 (-)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain ZYPA192
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3534824..3545972

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RBH03_RS16485 (RBH03_16490)	-	3535562..3536746 (-)	1185	WP_003110017.1	cystathionine gamma-synthase	-
RBH03_RS16490 (RBH03_16495)	-	3536743..3538116 (-)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
RBH03_RS16495 (RBH03_16500)	-	3538359..3538763 (-)	405	WP_003084560.1	NINE protein	-
RBH03_RS16500 (RBH03_16505)	czcD	3538917..3539816 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
RBH03_RS16505 (RBH03_16510)	pilU	3539824..3540972 (-)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
RBH03_RS16510 (RBH03_16515)	pilT	3541150..3542184 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
RBH03_RS16515 (RBH03_16520)	-	3542399..3543091 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
RBH03_RS16520 (RBH03_16525)	proC	3543103..3543924 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
RBH03_RS16525 (RBH03_16530)	-	3543935..3544528 (+)	594	WP_003084544.1	YggT family protein	-
RBH03_RS16530 (RBH03_16535)	-	3544525..3544821 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=871196 RBH03_RS16505 WP_003110016.1 3539824..3540972(-) (pilU) [Pseudomonas aeruginosa strain ZYPA192]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=871196 RBH03_RS16505 WP_003110016.1 3539824..3540972(-) (pilU) [Pseudomonas aeruginosa strain ZYPA192]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTTCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382