Detailed information NTG0870803

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RBB89_RS02115
• Genome accession: NZ_CP132949
• Coordinates: 460399..461547 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain IPA34
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 455399..466547

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RBB89_RS02090 (RBB89_02090)	-	456550..456846 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
RBB89_RS02095 (RBB89_02095)	-	456843..457436 (-)	594	WP_003084544.1	YggT family protein	-
RBB89_RS02100 (RBB89_02100)	proC	457447..458268 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
RBB89_RS02105 (RBB89_02105)	-	458280..458972 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
RBB89_RS02110 (RBB89_02110)	pilT	459187..460221 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
RBB89_RS02115 (RBB89_02115)	pilU	460399..461547 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
RBB89_RS02120 (RBB89_02120)	czcD	461555..462454 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
RBB89_RS02125 (RBB89_02125)	-	462608..463012 (+)	405	WP_003084560.1	NINE protein	-
RBB89_RS02130 (RBB89_02130)	-	463255..464628 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
RBB89_RS02135 (RBB89_02135)	-	464625..465809 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=870803 RBB89_RS02115 WP_003100959.1 460399..461547(+) (pilU) [Pseudomonas aeruginosa strain IPA34]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=870803 RBB89_RS02115 WP_003100959.1 460399..461547(+) (pilU) [Pseudomonas aeruginosa strain IPA34]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382