Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   LACPH_RS02435 Genome accession   NZ_CP132482
Coordinates   520013..520450 (+) Length   145 a.a.
NCBI ID   WP_306387211.1    Uniprot ID   -
Organism   Lacticaseibacillus parahuelsenbergensis strain NCIMB 15471     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 512044..555246 520013..520450 within 0


Gene organization within MGE regions


Location: 512044..555246
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LACPH_RS02370 (LACPH_000474) - 512044..513213 (-) 1170 WP_306387677.1 site-specific integrase -
  LACPH_RS02375 (LACPH_000475) - 513540..514217 (-) 678 WP_204149148.1 transcriptional regulator -
  LACPH_RS02380 (LACPH_000476) - 514277..514951 (-) 675 WP_306387203.1 LexA family transcriptional regulator -
  LACPH_RS02385 (LACPH_000477) - 515113..515358 (+) 246 WP_306387204.1 helix-turn-helix transcriptional regulator -
  LACPH_RS02390 (LACPH_000478) - 515355..515567 (+) 213 WP_306387205.1 Bro-N domain-containing protein -
  LACPH_RS02395 (LACPH_000479) - 515669..516412 (+) 744 WP_306387206.1 phage antirepressor KilAC domain-containing protein -
  LACPH_RS02400 (LACPH_000480) - 516666..517256 (-) 591 WP_005688510.1 hypothetical protein -
  LACPH_RS02405 (LACPH_000481) - 517321..517677 (+) 357 WP_306387207.1 DUF771 domain-containing protein -
  LACPH_RS02410 (LACPH_000482) - 517762..517914 (+) 153 WP_005712709.1 hypothetical protein -
  LACPH_RS02415 (LACPH_000483) - 517919..518122 (+) 204 WP_306387208.1 hypothetical protein -
  LACPH_RS02420 (LACPH_000484) - 518139..518630 (+) 492 WP_061713682.1 siphovirus Gp157 family protein -
  LACPH_RS02425 (LACPH_000485) - 518642..519352 (+) 711 WP_306387209.1 ERF family protein -
  LACPH_RS02430 (LACPH_000486) - 519330..519998 (+) 669 WP_306387210.1 putative HNHc nuclease -
  LACPH_RS02435 (LACPH_000487) ssb 520013..520450 (+) 438 WP_306387211.1 single-stranded DNA-binding protein Machinery gene
  LACPH_RS02440 (LACPH_000488) - 520467..521294 (+) 828 WP_306387678.1 helix-turn-helix domain-containing protein -
  LACPH_RS02445 (LACPH_000489) - 521291..522553 (+) 1263 WP_306387212.1 DnaB-like helicase C-terminal domain-containing protein -
  LACPH_RS02450 (LACPH_000490) - 522555..522899 (+) 345 WP_284794981.1 hypothetical protein -
  LACPH_RS02455 (LACPH_000491) - 522941..523342 (+) 402 WP_306387213.1 RusA family crossover junction endodeoxyribonuclease -
  LACPH_RS02460 (LACPH_000492) - 523352..524062 (+) 711 WP_306387214.1 N-6 DNA methylase -
  LACPH_RS02465 (LACPH_000493) - 524108..524689 (+) 582 WP_306387215.1 site-specific integrase -
  LACPH_RS02470 (LACPH_000494) - 524699..525277 (+) 579 WP_306387216.1 hypothetical protein -
  LACPH_RS02475 (LACPH_000495) - 525301..525411 (+) 111 WP_306387217.1 acetyltransferase -
  LACPH_RS02480 (LACPH_000496) - 525408..525929 (+) 522 WP_306387218.1 DUF1642 domain-containing protein -
  LACPH_RS02485 (LACPH_000497) - 525922..526476 (+) 555 WP_306387219.1 NUMOD4 domain-containing protein -
  LACPH_RS02490 (LACPH_000498) - 526469..526597 (+) 129 WP_306387220.1 hypothetical protein -
  LACPH_RS02495 (LACPH_000499) - 526587..526970 (+) 384 WP_138117716.1 DUF1642 domain-containing protein -
  LACPH_RS02500 (LACPH_000500) - 526967..527359 (+) 393 WP_306387221.1 YopX family protein -
  LACPH_RS02505 (LACPH_000501) - 527356..527643 (+) 288 WP_138117718.1 hypothetical protein -
  LACPH_RS02510 (LACPH_000502) - 527993..528436 (+) 444 WP_306387222.1 ArpU family phage packaging/lysis transcriptional regulator -
  LACPH_RS02515 (LACPH_000503) - 528748..529455 (+) 708 WP_204149130.1 hypothetical protein -
  LACPH_RS02520 (LACPH_000504) - 529687..529905 (-) 219 WP_025013983.1 CsbD family protein -
  LACPH_RS02525 (LACPH_000505) - 530312..531460 (+) 1149 WP_306387223.1 GcrA family cell cycle regulator -
  LACPH_RS02530 (LACPH_000506) - 531453..531776 (+) 324 WP_306387224.1 ribonucleoside-diphosphate reductase -
  LACPH_RS02535 (LACPH_000507) - 531779..532096 (+) 318 WP_306387225.1 hypothetical protein -
  LACPH_RS02540 (LACPH_000508) - 532247..533047 (+) 801 WP_306387226.1 HNH endonuclease -
  LACPH_RS02545 (LACPH_000509) - 533251..533706 (+) 456 WP_306387227.1 P27 family phage terminase small subunit -
  LACPH_RS02550 (LACPH_000510) - 533728..535440 (+) 1713 WP_306387228.1 terminase large subunit -
  LACPH_RS02555 (LACPH_000511) - 535452..535643 (+) 192 WP_003661399.1 hypothetical protein -
  LACPH_RS02560 (LACPH_000512) - 535649..536902 (+) 1254 WP_306387229.1 phage portal protein -
  LACPH_RS02565 (LACPH_000513) - 536856..537485 (+) 630 WP_306387230.1 HK97 family phage prohead protease -
  LACPH_RS02570 (LACPH_000514) - 537527..538729 (+) 1203 WP_306387231.1 phage major capsid protein -
  LACPH_RS02575 (LACPH_000515) - 538747..538986 (+) 240 WP_306387232.1 Ig-like domain-containing protein -
  LACPH_RS02580 (LACPH_000516) - 538997..539356 (+) 360 WP_306387233.1 head-tail connector protein -
  LACPH_RS02585 (LACPH_000517) - 539346..539675 (+) 330 WP_306387234.1 head-tail adaptor protein -
  LACPH_RS02590 (LACPH_000518) - 539675..540061 (+) 387 WP_306387235.1 HK97-gp10 family putative phage morphogenesis protein -
  LACPH_RS02595 (LACPH_000519) - 540061..540447 (+) 387 WP_306387236.1 phage tail protein -
  LACPH_RS02600 (LACPH_000520) - 540481..541089 (+) 609 WP_087911794.1 major tail protein -
  LACPH_RS02605 (LACPH_000521) - 541143..541331 (+) 189 WP_236944229.1 Ig-like domain-containing protein -
  LACPH_RS02610 (LACPH_000522) gpG 541402..541815 (+) 414 WP_003661382.1 phage tail assembly chaperone G -
  LACPH_RS02615 (LACPH_000523) - 541938..546800 (+) 4863 WP_306387237.1 phage tail tape measure protein -
  LACPH_RS02620 (LACPH_000524) - 546801..548861 (+) 2061 WP_306387238.1 distal tail protein Dit -
  LACPH_RS02625 (LACPH_000525) - 548862..551297 (+) 2436 WP_306387239.1 phage tail protein -
  LACPH_RS02630 (LACPH_000526) - 551299..551589 (+) 291 WP_306387240.1 hypothetical protein -
  LACPH_RS02635 (LACPH_000527) - 551582..551713 (+) 132 WP_019888587.1 XkdX family protein -
  LACPH_RS02640 (LACPH_000528) - 551743..552033 (+) 291 WP_306387241.1 hypothetical protein -
  LACPH_RS02645 (LACPH_000529) - 552026..552361 (+) 336 Protein_527 phage holin -
  LACPH_RS02650 (LACPH_000530) - 552649..552921 (+) 273 WP_070543981.1 DUF6275 family protein -
  LACPH_RS02655 (LACPH_000531) - 552933..554096 (+) 1164 WP_306387242.1 GH25 family lysozyme -
  LACPH_RS02660 (LACPH_000532) - 554461..555246 (+) 786 WP_274784406.1 hypothetical protein -

Sequence


Protein


Download         Length: 145 a.a.        Molecular weight: 15916.45 Da        Isoelectric Point: 4.8434

>NTDB_id=869181 LACPH_RS02435 WP_306387211.1 520013..520450(+) (ssb) [Lacticaseibacillus parahuelsenbergensis strain NCIMB 15471]
MLNSVALTGRLTKDVDLRYTQSGTAVGSFTIAVDRQFRSANGNRDTDFINCAIWRKSAENFANFTHKGSLVGIEGHIQTR
TYDNAQGQKVFVTEVIVENFALLEPRQMSQDSQQRSDNNPAAASQGNGFANNGQPVDVSDDDLPF

Nucleotide


Download         Length: 438 bp        

>NTDB_id=869181 LACPH_RS02435 WP_306387211.1 520013..520450(+) (ssb) [Lacticaseibacillus parahuelsenbergensis strain NCIMB 15471]
ATGCTTAATTCAGTTGCTTTAACAGGCAGATTAACTAAAGACGTTGACCTTCGCTACACACAAAGCGGAACGGCAGTCGG
TTCATTCACAATCGCTGTTGACCGTCAATTCCGCAGTGCGAACGGTAACCGGGATACTGACTTCATCAATTGTGCCATCT
GGCGTAAGTCTGCTGAGAATTTTGCCAACTTTACGCACAAGGGTTCACTCGTTGGCATCGAAGGTCATATCCAAACACGT
ACGTACGATAACGCGCAAGGCCAGAAAGTATTCGTGACTGAGGTCATTGTTGAGAATTTTGCTTTGCTTGAGCCACGGCA
GATGTCTCAGGACAGCCAACAGCGATCGGATAATAACCCAGCGGCCGCAAGCCAAGGCAACGGTTTTGCTAACAATGGCC
AGCCAGTCGATGTCAGCGATGATGATCTTCCATTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

56.471

100

0.662

  ssbA Bacillus subtilis subsp. subtilis str. 168

47.093

100

0.559

  ssbB Bacillus subtilis subsp. subtilis str. 168

51.887

73.103

0.379

  ssbB Streptococcus sobrinus strain NIDR 6715-7

37.241

100

0.372