Detailed information NTG0868215

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RAL92_RS09925
• Genome accession: NZ_CP132337
• Coordinates: 2125136..2126350 (+)
• Length: 404 a.a.
• NCBI ID: WP_174244726.1
• Uniprot ID: -
• Organism: Pseudomonas otitidis strain Tg_9B
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2120136..2131350

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RAL92_RS09900 (RAL92_09905)	-	2120271..2120660 (-)	390	WP_346842128.1	GtrA family protein	-
RAL92_RS09905 (RAL92_09910)	-	2120657..2121658 (-)	1002	WP_301153625.1	glycosyltransferase family 2 protein	-
RAL92_RS09910 (RAL92_09915)	-	2121651..2123144 (-)	1494	WP_346842129.1	glycosyltransferase family 39 protein	-
RAL92_RS09915 (RAL92_09920)	-	2123288..2124595 (-)	1308	WP_346842130.1	PAS domain-containing methyl-accepting chemotaxis protein	-
RAL92_RS09920 (RAL92_09925)	-	2124714..2124992 (-)	279	WP_044409048.1	peptidylprolyl isomerase	-
RAL92_RS09925 (RAL92_09930)	pilU	2125136..2126350 (+)	1215	WP_174244726.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RAL92_RS09930 (RAL92_09935)	-	2126379..2127113 (-)	735	WP_282887987.1	transporter substrate-binding domain-containing protein	-
RAL92_RS09935 (RAL92_09940)	-	2127199..2127924 (-)	726	WP_346842131.1	alpha/beta fold hydrolase	-
RAL92_RS09940 (RAL92_09945)	-	2127905..2128696 (-)	792	WP_044409054.1	crotonase/enoyl-CoA hydratase family protein	-
RAL92_RS09945 (RAL92_09950)	-	2129049..2130725 (+)	1677	WP_044409057.1	DEAD/DEAH box helicase	-

Sequence

Protein

• Length: 404 a.a.
• Molecular weight: 44460.86 Da
• Isoelectric Point: 6.6002

>NTDB_id=868215 RAL92_RS09925 WP_174244726.1 2125136..2126350(+) (pilU) [Pseudomonas otitidis strain Tg_9B]
MDLPSMLKVLASQDGSDLYLSTGAPPCAKFNGVLKPLSADPLKPGDVARIAESIMDPEQRVEFNHELEMNLAISVPNVGR
FRINIFKQRNEVSIVARNIKLDIPRFEDLKLPEVLLKVVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTSDGKRRAAVEVLLGTSTISDLIKRGDFSSIKEIMEKSKALGMQTFDQAL
FDLVVEGAISEDEAIKNADSANNLRLKLKLYRDGPAPAPTPAPAAAPTPPPAATRTQDAASWGLELKLEELPEETPPEDP
GKHF

Nucleotide

• Length: 1215 bp

>NTDB_id=868215 RAL92_RS09925 WP_174244726.1 2125136..2126350(+) (pilU) [Pseudomonas otitidis strain Tg_9B]
ATGGATCTCCCTTCGATGCTGAAGGTCCTTGCCTCCCAGGATGGCTCGGACCTCTACCTGTCCACCGGCGCGCCGCCCTG
CGCCAAGTTCAATGGCGTGCTCAAGCCGCTGAGCGCCGACCCGCTCAAGCCGGGGGACGTGGCGCGTATCGCCGAGTCGA
TCATGGACCCGGAGCAGCGCGTCGAATTCAACCATGAGCTGGAAATGAACCTGGCGATCTCGGTGCCCAACGTGGGGCGC
TTCCGCATCAACATCTTCAAGCAGCGCAACGAGGTCTCCATCGTTGCGCGCAACATCAAGCTGGACATCCCCCGCTTCGA
GGACCTCAAGCTGCCCGAGGTGCTGCTCAAGGTGGTGATGGAGAAGCGCGGCCTGGTGCTCTTCGTCGGCGGCACCGGCT
CCGGCAAGTCCACCTCCCTGGCGGCGCTGATCGACTACCGCAACCGCAACAGCGGCGGGCACATCATCACCATCGAGGAC
CCGGTGGAGTACGTGCACCGGCACAAGAAATCCATCATCAACCAGCGCGAGGTGGGGGTGGACACCCGCAGCTTCCACGC
GGCGTTGAAGAACACCCTGCGCCAGGCGCCCGACGTGATCCTCATTGGCGAGATCCGCGACCGAGAGACCATGGAGCACG
CCCTGGCCTTCGCCGACACCGGCCACCTGGCCATTTCCACCCTGCATGCGAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGGCCGCAGCTGCTCAACGACCTGGGCAACAACCTCAAGGCCTTCGTCTCCCAGCG
CCTGGTACGCACTTCCGATGGCAAGCGTCGCGCGGCGGTGGAGGTGCTGCTGGGCACTTCCACCATCAGCGACCTGATCA
AGCGCGGTGACTTCAGCTCCATCAAGGAGATCATGGAAAAGTCCAAGGCCCTGGGCATGCAGACCTTCGACCAGGCGCTG
TTCGACCTGGTGGTGGAAGGCGCCATCAGCGAGGACGAGGCGATCAAGAACGCCGACTCCGCCAACAACCTGCGCCTCAA
GCTGAAGCTCTACCGCGACGGCCCGGCTCCCGCACCGACGCCCGCTCCCGCCGCCGCACCCACCCCGCCACCAGCCGCGA
CCCGTACCCAGGACGCAGCCAGCTGGGGCCTGGAGCTGAAGCTGGAGGAGCTGCCCGAGGAAACGCCGCCGGAAGATCCG
GGCAAGCATTTCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (112-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.125	86.881	0.488
pilU	Acinetobacter baylyi ADP1	52.925	88.861	0.47
pilU	Vibrio cholerae strain A1552	52.125	87.376	0.455
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.83	93.069	0.361