Detailed information NTG0868204

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RAL92_RS01915
• Genome accession: NZ_CP132337
• Coordinates: 385547..386692 (+)
• Length: 381 a.a.
• NCBI ID: WP_044403589.1
• Uniprot ID: A0AB36F620
• Organism: Pseudomonas otitidis strain Tg_9B
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 380547..391692

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RAL92_RS01885 (RAL92_01885)	-	380636..380932 (-)	297	WP_044403598.1	DUF167 domain-containing protein	-
RAL92_RS01890 (RAL92_01890)	-	380932..381525 (-)	594	WP_074967194.1	YggT family protein	-
RAL92_RS01895 (RAL92_01895)	-	381617..382804 (-)	1188	WP_346841513.1	hypothetical protein	-
RAL92_RS01900 (RAL92_01900)	proC	382807..383625 (-)	819	WP_074967198.1	pyrroline-5-carboxylate reductase	-
RAL92_RS01905 (RAL92_01905)	-	383643..384335 (-)	693	WP_208274795.1	YggS family pyridoxal phosphate-dependent enzyme	-
RAL92_RS01910 (RAL92_01910)	pilT	384404..385438 (+)	1035	WP_142012818.1	type IV pilus twitching motility protein PilT	Machinery gene
RAL92_RS01915 (RAL92_01915)	pilU	385547..386692 (+)	1146	WP_044403589.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RAL92_RS01920	-	386634..388079 (-)	1446	WP_346841514.1	TIGR03915 family putative DNA repair protein	-
RAL92_RS01925 (RAL92_01930)	-	388079..389290 (-)	1212	WP_346841515.1	putative DNA modification/repair radical SAM protein	-
RAL92_RS01930 (RAL92_01935)	-	389397..389792 (+)	396	WP_074967204.1	TM2 domain-containing protein	-
RAL92_RS01935 (RAL92_01940)	-	389845..391116 (-)	1272	WP_346841516.1	dihydroorotase	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42485.80 Da
• Isoelectric Point: 6.7727

>NTDB_id=868204 RAL92_RS01915 WP_044403589.1 385547..386692(+) (pilU) [Pseudomonas otitidis strain Tg_9B]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKVMPVTKNPMSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEQLKLPEVLKKLAMTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEYIHQHQGCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQQQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLVADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYNLYSQGEITYEDALLYADSANDLRLMIKLGSETDGEHLTSISGGLSLEVSEDDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=868204 RAL92_RS01915 WP_044403589.1 385547..386692(+) (pilU) [Pseudomonas otitidis strain Tg_9B]
ATGGAATTCGAGAAACTGTTGCGCCTGATGGTCGAGAAGGGCGGTTCGGACCTGTTCATCACGGCCGGTGTGCCGCCGTC
GATGAAGGTCAACGGCAAGGTCATGCCGGTCACCAAGAACCCCATGTCGCCGGAGCAGACCCGGGAAACCGTGCTGGGGG
TGATGAACGAGCAGCAGCGCCGCGACTTCGCCGAGAACCACGAGTGCAACTTCGCCATCAGCGCCCGGGGCATCGGCCGT
TTCCGCGTCAGCGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGACCAACATCCCCACCCTGGA
GCAGCTCAAGCTGCCCGAGGTGCTGAAGAAGCTGGCCATGACCAAGCGCGGCCTGGTGCTCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACGTCCCTGGCGGCGATGATCGGCTACCGCAACAAGAACAGCTCCGGCCACATCATCTCCATCGAAGAC
CCCATCGAATACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGTGAAGTGGGGATCGACACCGAATCCTTCGAGGT
GGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAGGTGCGGACCCGCGAGACCATGGACCACG
CCGTGGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCCGCCGACCGTCAGCAGCAGGTGTGGATGGACCTGTCCCTCAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCGGATGGCAAGGGGCGCCGCGCGGTGATCGAGGTGCTGATCAACACCCCGCTGGTGGCCGACCTGA
TCCGCAAGGGCGAGGTCCACGAGCTCAAGCCGCTGATGAAACGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTACTCCCAGGGCGAGATCACCTACGAAGACGCCCTGCTCTACGCCGACTCGGCGAACGACCTGCGCCT
GATGATCAAGCTCGGTTCCGAGACCGACGGCGAACACCTGACCAGCATCAGTGGCGGGCTGTCCCTGGAAGTCTCGGAAG
ACGATCCGGGCCGTCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	92.126	100	0.921
pilU	Acinetobacter baylyi ADP1	63.866	93.701	0.598
pilU	Vibrio cholerae strain A1552	58.857	91.864	0.541
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.75	88.189	0.386