Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   B6N23_RS16890 Genome accession   NZ_CP131913
Coordinates   3591024..3592814 (-) Length   596 a.a.
NCBI ID   WP_305500943.1    Uniprot ID   -
Organism   Halomonas alkalicola strain CICC 11012s     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3586024..3597814
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B6N23_RS16865 (B6N23_16870) - 3586403..3587086 (+) 684 WP_305500930.1 TetR/AcrR family transcriptional regulator -
  B6N23_RS16870 (B6N23_16875) nagZ 3587231..3588238 (+) 1008 WP_305500933.1 beta-N-acetylhexosaminidase -
  B6N23_RS16875 (B6N23_16880) - 3588284..3588862 (+) 579 WP_305500937.1 hypoxanthine-guanine phosphoribosyltransferase -
  B6N23_RS16880 (B6N23_16885) pilD 3588866..3589714 (-) 849 WP_305503882.1 A24 family peptidase Machinery gene
  B6N23_RS16885 (B6N23_16890) pilC 3589736..3590971 (-) 1236 WP_305500941.1 type II secretion system F family protein Machinery gene
  B6N23_RS16890 (B6N23_16895) pilB 3591024..3592814 (-) 1791 WP_305500943.1 type IV-A pilus assembly ATPase PilB Machinery gene
  B6N23_RS16895 (B6N23_16900) - 3593035..3593445 (-) 411 WP_110070079.1 DUF86 domain-containing protein -
  B6N23_RS16900 (B6N23_16905) - 3593435..3593860 (-) 426 WP_305500946.1 nucleotidyltransferase domain-containing protein -
  B6N23_RS16905 (B6N23_16910) - 3593982..3594125 (-) 144 WP_305500948.1 hypothetical protein -
  B6N23_RS16910 (B6N23_16915) - 3594382..3595824 (+) 1443 WP_305500950.1 MBL fold metallo-hydrolase RNA specificity domain-containing protein -
  B6N23_RS16915 (B6N23_16920) - 3595836..3596267 (+) 432 WP_305500952.1 low molecular weight protein-tyrosine-phosphatase -
  B6N23_RS16920 (B6N23_16925) - 3596452..3597672 (+) 1221 WP_305500955.1 transposase -

Sequence

Protein


Download         Length: 596 a.a.        Molecular weight: 64979.67 Da        Isoelectric Point: 5.7916

>NTDB_id=866112 B6N23_RS16890 WP_305500943.1 3591024..3592814(-) (pilB) [Halomonas alkalicola strain CICC 11012s]
MNDYRSPPSAPPLDTPLQSTRHDATAGLRGLARRLVADERISHAAAVRAEEEAREGEVSLLEHVIDTGLVSARQATLSAA
WEYGLPMMDLDAIRPETLPRADAFPEKILRKLKVLPLMQRGHRLTVAVPYPSTLALLDELQFATGLTPEGVLAPVDQLGP
VLEAYLASSEKSMLDELKGVDDAIGELGFAGEEEASGEVDVQAAASAASEDAPIIKFVNKILLDAIHRGASDIHFEPYET
SYRVRFRIDGMLHDVAHPPFAMRARIAARLKVMSRLDISERRLPQDGAIKLRLSKTRSIDFRVNSLPTVFGEKIVLRILD
PASAQLGIEMLGFTPEQRAMYETVLDQPQGMILVTGPTGSGKTVTLYTGINILNEVERNICTAEDPVEIKVPGVNQVNVI
PKIGLDFASALRAFLRQDPDVVMVGEIRDLETAEIAVKASQTGHLVLSTVHTNSAAETLTRLANMGLAPFNIASSVSLII
AQRLARKLCKHCKQPADLPHEALAAQGFTEEEIGQATFFEPVGCKHCTKGFKGRVGIYEVVPISHAMSQLIMKNGNSMEF
SDLARQEGYPDLRRSGLMKAMQGVTSLAEINRVTKD

Nucleotide


Download         Length: 1791 bp        

>NTDB_id=866112 B6N23_RS16890 WP_305500943.1 3591024..3592814(-) (pilB) [Halomonas alkalicola strain CICC 11012s]
ATGAACGACTATCGCAGCCCACCGAGTGCCCCTCCTCTGGACACCCCACTACAAAGCACCCGCCACGACGCCACGGCGGG
CCTGCGCGGTCTGGCGCGGCGACTGGTGGCGGACGAGCGGATCAGCCACGCCGCCGCGGTGCGCGCTGAGGAGGAGGCCC
GCGAGGGCGAGGTGTCGCTGCTGGAGCACGTGATCGATACCGGGCTGGTCTCCGCCCGCCAGGCCACCCTCTCGGCGGCT
TGGGAGTATGGCTTGCCGATGATGGATCTCGACGCCATCCGCCCGGAGACGCTGCCCCGCGCCGACGCCTTCCCCGAAAA
GATCCTGCGCAAGCTCAAGGTGCTGCCGCTGATGCAGCGCGGCCACCGGCTCACCGTGGCGGTGCCCTACCCCTCTACGC
TTGCCCTGCTCGACGAGCTGCAGTTCGCCACCGGCCTCACTCCGGAGGGGGTGCTGGCCCCGGTGGACCAGCTCGGCCCG
GTGTTGGAGGCCTATCTCGCCTCCAGTGAGAAGAGCATGCTCGATGAGCTCAAGGGGGTGGACGATGCCATCGGCGAGCT
GGGCTTCGCCGGCGAGGAGGAGGCCAGCGGCGAGGTAGACGTGCAGGCGGCCGCGAGTGCGGCCAGCGAAGACGCGCCGA
TCATCAAGTTCGTCAACAAGATCCTGCTCGACGCCATCCACCGCGGCGCCTCGGACATCCACTTCGAGCCCTATGAAACC
AGCTACCGGGTGCGCTTTCGCATCGACGGCATGCTGCATGACGTGGCCCACCCGCCCTTCGCCATGCGCGCGCGCATCGC
CGCGCGCCTGAAGGTGATGTCACGGCTCGATATCTCGGAGCGCCGCCTGCCCCAGGACGGCGCCATCAAGCTCCGGCTCT
CGAAGACCCGCTCCATCGACTTTCGCGTCAACTCGCTGCCCACGGTGTTCGGCGAGAAGATCGTGTTGCGGATCCTCGAC
CCCGCCTCGGCCCAGCTCGGCATCGAGATGCTCGGCTTCACGCCGGAGCAGCGCGCCATGTACGAGACGGTGCTGGATCA
GCCCCAGGGCATGATTCTCGTCACCGGGCCCACCGGCAGCGGCAAGACGGTGACCCTCTATACCGGCATCAATATCCTCA
ACGAGGTGGAGCGCAACATCTGCACCGCCGAGGACCCGGTAGAGATCAAGGTGCCCGGGGTCAACCAGGTCAACGTGATC
CCCAAGATCGGCCTGGACTTCGCCAGCGCCCTGCGTGCCTTCCTGCGCCAGGACCCGGACGTGGTGATGGTGGGCGAGAT
CCGCGACCTGGAGACCGCCGAGATCGCGGTGAAGGCCTCCCAGACCGGCCACCTGGTGCTCTCCACCGTGCACACTAACT
CCGCTGCGGAGACCCTCACCCGCCTGGCCAACATGGGCCTGGCGCCGTTCAATATCGCCAGTTCCGTGAGTCTGATCATC
GCCCAGCGCCTGGCGCGCAAGCTCTGCAAGCACTGCAAGCAGCCGGCGGACCTGCCCCACGAGGCCCTGGCCGCCCAAGG
CTTCACGGAGGAGGAGATCGGCCAGGCCACTTTCTTCGAGCCGGTGGGCTGCAAGCACTGCACCAAGGGCTTCAAGGGGC
GGGTGGGCATCTATGAGGTGGTGCCGATCAGCCACGCCATGAGTCAATTGATCATGAAGAATGGCAATTCCATGGAGTTC
AGCGACCTGGCCCGCCAGGAGGGCTATCCGGACCTGCGCAGAAGCGGCCTCATGAAGGCGATGCAGGGGGTGACCAGCCT
GGCCGAGATCAACAGGGTGACCAAAGACTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(67-169)

Type II/IV secretion system protein (T2SSE)

(220-487)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

51.91

96.644

0.502
  pilB Acinetobacter baumannii D1279779

51.73

96.98

0.502
  pilB Legionella pneumophila strain ERS1305867

52.364

95.805

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

48.825

92.785

0.453
  pilB Vibrio campbellii strain DS40M4

49.36

91.779

0.453
  pilB Vibrio cholerae strain A1552

49.813

89.933

0.448
  pilF Neisseria gonorrhoeae MS11

45.359

95.805

0.435
  pilB/pilB1 Synechocystis sp. PCC 6803

39.162

92.114

0.361