Detailed information NTG0866013

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: WHO29_RS09365
• Genome accession: NZ_CP148133
• Coordinates: 1938351..1939361 (+)
• Length: 336 a.a.
• NCBI ID: WP_000568519.1
• Uniprot ID: Q32HA1
• Organism: Escherichia coli isolate FELIX_MS78
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1933351..1944361

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WHO29_RS09335	nudB	1935075..1935527 (+)	453	WP_001300367.1	dihydroneopterin triphosphate diphosphatase	-
WHO29_RS09340	yebC	1935556..1936296 (+)	741	WP_000907248.1	YebC/PmpR family DNA-binding transcriptional regulator	-
WHO29_RS09345	ruvC	1936331..1936852 (+)	522	WP_001295503.1	crossover junction endodeoxyribonuclease RuvC	-
WHO29_RS09350	yebB	1936854..1937456 (-)	603	WP_001024932.1	YebB family permuted papain-like enzyme	-
WHO29_RS09355	yobI	1937527..1937592 (+)	66	WP_010723105.1	stress response small protein YobI	-
WHO29_RS09360	ruvA	1937731..1938342 (+)	612	WP_000580323.1	Holliday junction branch migration protein RuvA	-
WHO29_RS09365	ruvB	1938351..1939361 (+)	1011	WP_000568519.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
WHO29_RS09370	znuB	1939508..1940293 (-)	786	WP_000571480.1	zinc ABC transporter permease subunit ZnuB	-
WHO29_RS09375	znuC	1940290..1941045 (-)	756	WP_000202996.1	zinc ABC transporter ATP-binding protein ZnuC	-
WHO29_RS09380	znuA	1941124..1942056 (+)	933	WP_001300644.1	zinc ABC transporter substrate-binding protein ZnuA	-
WHO29_RS09385	mepM	1942072..1943394 (+)	1323	WP_001184045.1	murein DD-endopeptidase MepM	-

Sequence

Protein

• Length: 336 a.a.
• Molecular weight: 37173.77 Da
• Isoelectric Point: 4.7818

>NTDB_id=866013 WHO29_RS09365 WP_000568519.1 1938351..1939361(+) (ruvB) [Escherichia coli isolate FELIX_MS78]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide

• Length: 1011 bp

>NTDB_id=866013 WHO29_RS09365 WP_000568519.1 1938351..1939361(+) (ruvB) [Escherichia coli isolate FELIX_MS78]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTAGCAGATCGCGCCATTCGCCCCAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATTTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATTTGGCTGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTCTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGACCTGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (23-181)

• RuvB AAA lid domain (AAA_lid_4) (184-257)

• RuvB C-terminal winged helix domain (WHD_RuvB) (259-328)

3D structure

• AlphaFold DB: Q32HA1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	60.486	97.917	0.592
ruvB	Streptococcus pneumoniae TIGR4	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae R6	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae D39	58.934	94.94	0.56
ruvB	Synechocystis sp. PCC 6803	53.251	96.131	0.512
ruvB	Helicobacter pylori 26695	52.038	94.94	0.494