Detailed information NTG0865932

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: WHO52_RS09360
• Genome accession: NZ_CP148132
• Coordinates: 1937597..1938607 (+)
• Length: 336 a.a.
• NCBI ID: WP_000568519.1
• Uniprot ID: Q32HA1
• Organism: Escherichia coli isolate FELIX_MS75
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1932597..1943607

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WHO52_RS09330	nudB	1934321..1934773 (+)	453	WP_001300367.1	dihydroneopterin triphosphate diphosphatase	-
WHO52_RS09335	yebC	1934802..1935542 (+)	741	WP_000907248.1	YebC/PmpR family DNA-binding transcriptional regulator	-
WHO52_RS09340	ruvC	1935577..1936098 (+)	522	WP_001295503.1	crossover junction endodeoxyribonuclease RuvC	-
WHO52_RS09345	yebB	1936100..1936702 (-)	603	WP_001024932.1	YebB family permuted papain-like enzyme	-
WHO52_RS09350	yobI	1936773..1936838 (+)	66	WP_010723105.1	stress response small protein YobI	-
WHO52_RS09355	ruvA	1936977..1937588 (+)	612	WP_000580323.1	Holliday junction branch migration protein RuvA	-
WHO52_RS09360	ruvB	1937597..1938607 (+)	1011	WP_000568519.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
WHO52_RS09365	znuB	1938754..1939539 (-)	786	WP_000571480.1	zinc ABC transporter permease subunit ZnuB	-
WHO52_RS09370	znuC	1939536..1940291 (-)	756	WP_000202996.1	zinc ABC transporter ATP-binding protein ZnuC	-
WHO52_RS09375	znuA	1940370..1941302 (+)	933	WP_001300644.1	zinc ABC transporter substrate-binding protein ZnuA	-
WHO52_RS09380	mepM	1941318..1942640 (+)	1323	WP_001184045.1	murein DD-endopeptidase MepM	-

Sequence

Protein

• Length: 336 a.a.
• Molecular weight: 37173.77 Da
• Isoelectric Point: 4.7818

>NTDB_id=865932 WHO52_RS09360 WP_000568519.1 1937597..1938607(+) (ruvB) [Escherichia coli isolate FELIX_MS75]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide

• Length: 1011 bp

>NTDB_id=865932 WHO52_RS09360 WP_000568519.1 1937597..1938607(+) (ruvB) [Escherichia coli isolate FELIX_MS75]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTAGCAGATCGCGCCATTCGCCCCAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATTTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATTTGGCTGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTCTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGACCTGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (23-181)

• RuvB AAA lid domain (AAA_lid_4) (184-257)

• RuvB C-terminal winged helix domain (WHD_RuvB) (259-328)

3D structure

• AlphaFold DB: Q32HA1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	60.486	97.917	0.592
ruvB	Streptococcus pneumoniae TIGR4	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae R6	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae D39	58.934	94.94	0.56
ruvB	Synechocystis sp. PCC 6803	53.251	96.131	0.512
ruvB	Helicobacter pylori 26695	52.038	94.94	0.494