Detailed information NTG0865725

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: Q7572_RS14230
• Genome accession: NZ_CP131878
• Coordinates: 2944226..2945344 (+)
• Length: 372 a.a.
• NCBI ID: WP_002052019.1
• Uniprot ID: A0A1V0YUM2
• Organism: Acinetobacter nosocomialis strain 2014S01-097
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2939226..2950344

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q7572_RS14215 (Q7572_14245)	-	2941393..2942235 (-)	843	WP_004888364.1	undecaprenyl-diphosphate phosphatase	-
Q7572_RS14220 (Q7572_14250)	-	2942342..2943034 (-)	693	WP_004888366.1	YggS family pyridoxal phosphate-dependent enzyme	-
Q7572_RS14225 (Q7572_14255)	pilT	2943161..2944198 (+)	1038	WP_006581387.1	type IV pilus twitching motility protein PilT	Machinery gene
Q7572_RS14230 (Q7572_14260)	pilU	2944226..2945344 (+)	1119	WP_002052019.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
Q7572_RS14235 (Q7572_14265)	fur	2945414..2945851 (-)	438	WP_002052029.1	ferric iron uptake transcriptional regulator	-
Q7572_RS14240 (Q7572_14270)	bamE	2945963..2946361 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
Q7572_RS14245 (Q7572_14275)	-	2946417..2946740 (-)	324	WP_004888367.1	RnfH family protein	-
Q7572_RS14250 (Q7572_14280)	-	2946740..2947807 (-)	1068	WP_151277455.1	hypothetical protein	-
Q7572_RS14255 (Q7572_14285)	-	2947960..2948415 (-)	456	WP_000782976.1	bacteriohemerythrin	-
Q7572_RS14260 (Q7572_14290)	-	2948571..2949393 (+)	823	WP_076611894.1	IS5-like element ISAba27 family transposase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41980.18 Da
• Isoelectric Point: 6.6944

>NTDB_id=865725 Q7572_RS14230 WP_002052019.1 2944226..2945344(+) (pilU) [Acinetobacter nosocomialis strain 2014S01-097]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPTVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLANEGPDQITDEKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=865725 Q7572_RS14230 WP_002052019.1 2944226..2945344(+) (pilU) [Acinetobacter nosocomialis strain 2014S01-097]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCCGATCTGTTTATTACAGATGGTGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGCCTTTCAGGTGAAGTGATTGGACAGCTCTTACATTCCA
TCATGAGTGAAAAACAACGTAAAGAATTTGCAGAGACTCGTGAATGTAACTTTGCCATCATGAACCGCGATAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAACGTGACATGCCGGGTATGGTACTACGTCGAATTGAAACGAAAATTCCTTCAAT
TGATGACTTACAGTTGCCGACTGTGCTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGTGCAACAG
GTACAGGTAAATCGACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGCCATATCATTACCATTGAG
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAACGTGAAGTCGGTATTGATACAGACTCATTTGA
AATTGCTTTAAAAAATACGTTACGACAAGCGCCAGATGTCATCTTAATTGGTGAAATCCGTTCTCGCGAAGTCATGGACT
ACGCAATTGGCTTTGCTGAAACAGGACACCTTGTATTAGCTACAATGCACGCCAACAACGCCAACCAAGCACTCGACCGT
ATTATTCACTTTTTCGAAAGTGACCGTCATAGCCAGCTTTACATGGACTTGTCTCTTAACTTAAAAGCCATGATTGCACA
GCAGCTTATTCCAACACCAGATGGTAATTCACGCCGTGCAGCGATTGAGATCTTAATTAACTCACCATTAATTTCAGACT
ATATCCGTAAAGGTGAAATTCATGAAATTAAGGACTTGATGAAACGCTCACGTGAGCTTGGTATGCAGACCTTTGACCAA
GCTTTATTCGACTTATATAAAGCAGGCCAAATTACTTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTAACGATTAAGCTTGCAAATGAAGGGCCTGATCAAATAACAGACGAAAAACAGCATTTAACTTTTGATCGTCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

3D structure

• AlphaFold DB: A0A1V0YUM2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.464	99.731	0.683
pilU	Vibrio cholerae strain A1552	53.514	99.462	0.532
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.254	95.43	0.403