Detailed information NTG0865192

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: Q7585_RS01990
• Genome accession: NZ_CP131794
• Coordinates: 430121..431269 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain 2014S01-136
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 425121..436269

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q7585_RS01965 (Q7585_01965)	-	426272..426568 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
Q7585_RS01970 (Q7585_01970)	-	426565..427158 (-)	594	WP_003084544.1	YggT family protein	-
Q7585_RS01975 (Q7585_01975)	proC	427169..427990 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
Q7585_RS01980 (Q7585_01980)	-	428002..428694 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
Q7585_RS01985 (Q7585_01985)	pilT	428909..429943 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
Q7585_RS01990 (Q7585_01990)	pilU	430121..431269 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
Q7585_RS01995 (Q7585_01995)	czcD	431277..432176 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
Q7585_RS02000 (Q7585_02000)	-	432330..432734 (+)	405	WP_003084560.1	NINE protein	-
Q7585_RS02005 (Q7585_02005)	-	432977..434350 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
Q7585_RS02010 (Q7585_02010)	-	434347..435531 (+)	1185	WP_015649763.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=865192 Q7585_RS01990 WP_003100959.1 430121..431269(+) (pilU) [Pseudomonas aeruginosa strain 2014S01-136]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=865192 Q7585_RS01990 WP_003100959.1 430121..431269(+) (pilU) [Pseudomonas aeruginosa strain 2014S01-136]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATAGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382