Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   WAC27_RS06850 Genome accession   NZ_CP148034
Coordinates   1389268..1390506 (-) Length   412 a.a.
NCBI ID   WP_000815984.1    Uniprot ID   -
Organism   Escherichia coli strain NH-21     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1384268..1395506
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WAC27_RS06830 (WAC27_06830) dam 1384880..1385716 (-) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -
  WAC27_RS06835 (WAC27_06835) damX 1385823..1387109 (-) 1287 WP_000343195.1 cell division protein DamX -
  WAC27_RS06840 (WAC27_06840) aroB 1387201..1388289 (-) 1089 WP_000439850.1 3-dehydroquinate synthase -
  WAC27_RS06845 (WAC27_06845) aroK 1388346..1388867 (-) 522 WP_000818618.1 shikimate kinase AroK -
  WAC27_RS06850 (WAC27_06850) comE 1389268..1390506 (-) 1239 WP_000815984.1 DNA uptake porin HofQ Machinery gene
  WAC27_RS06855 (WAC27_06855) hofP 1390418..1390822 (-) 405 WP_001338740.1 DNA utilization protein HofP -
  WAC27_RS06860 (WAC27_06860) hofO 1390812..1391252 (-) 441 WP_001055768.1 DNA utilization protein HofO -
  WAC27_RS06865 (WAC27_06865) hofN 1391236..1391775 (-) 540 WP_001069320.1 DNA utilization protein HofN -
  WAC27_RS06870 (WAC27_06870) hofM 1391775..1392554 (-) 780 WP_001338738.1 DNA utilization protein HofM -
  WAC27_RS06875 (WAC27_06875) mrcA 1392674..1395226 (+) 2553 WP_001300707.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44643.18 Da        Isoelectric Point: 6.3183

>NTDB_id=863650 WAC27_RS06850 WP_000815984.1 1389268..1390506(-) (comE) [Escherichia coli strain NH-21]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSIAWQNNNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPLFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=863650 WAC27_RS06850 WP_000815984.1 1389268..1390506(-) (comE) [Escherichia coli strain NH-21]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAAGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGCCAGGAGGGCAACATTCTCTCAGTGCATTCCATTGCCTGGCAGAATAACAATATCGCCCGCCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC
AAAACGGCGTTAAGCGCACTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACACGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGTAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAAC
GGACGTTTGCTGGATCTTGAGCTTTCTGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCTAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCTGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTTGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  comE Haemophilus influenzae 86-028NP

36.927

100

0.391
  pilQ Vibrio campbellii strain DS40M4

38.242

100

0.391
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.53

100

0.376
  pilQ Vibrio cholerae strain A1552

37.53

100

0.376
  comE Glaesserella parasuis strain SC1401

35.952

100

0.367