Detailed information NTG0862740

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: Q6D61_RS02390
• Genome accession: NZ_CP131614
• Coordinates: 487397..488545 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain 1709-25403
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 482397..493545

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q6D61_RS02365 (Q6D61_02365)	-	483548..483844 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
Q6D61_RS02370 (Q6D61_02370)	-	483841..484434 (-)	594	WP_003084544.1	YggT family protein	-
Q6D61_RS02375 (Q6D61_02375)	proC	484445..485266 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
Q6D61_RS02380 (Q6D61_02380)	-	485278..485970 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
Q6D61_RS02385 (Q6D61_02385)	pilT	486185..487219 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
Q6D61_RS02390 (Q6D61_02390)	pilU	487397..488545 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
Q6D61_RS02395 (Q6D61_02395)	czcD	488553..489452 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
Q6D61_RS02400 (Q6D61_02400)	-	489606..490010 (+)	405	WP_003084560.1	NINE protein	-
Q6D61_RS02405 (Q6D61_02405)	-	490253..491626 (+)	1374	WP_034039850.1	pyridoxal-phosphate dependent enzyme	-
Q6D61_RS02410 (Q6D61_02410)	-	491623..492807 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=862740 Q6D61_RS02390 WP_003100959.1 487397..488545(+) (pilU) [Pseudomonas aeruginosa strain 1709-25403]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=862740 Q6D61_RS02390 WP_003100959.1 487397..488545(+) (pilU) [Pseudomonas aeruginosa strain 1709-25403]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382