Detailed information NTG0862246

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: Q7F19_RS31205
• Genome accession: NZ_CP130942
• Coordinates: 6632367..6633515 (-)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain 2023CK-00048
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6627367..6638515

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q7F19_RS31185 (Q7F19_31185)	-	6628105..6629289 (-)	1185	WP_003110017.1	cystathionine gamma-synthase	-
Q7F19_RS31190 (Q7F19_31190)	-	6629286..6630659 (-)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
Q7F19_RS31195 (Q7F19_31195)	-	6630902..6631306 (-)	405	WP_003084560.1	NINE protein	-
Q7F19_RS31200 (Q7F19_31200)	czcD	6631460..6632359 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
Q7F19_RS31205 (Q7F19_31205)	pilU	6632367..6633515 (-)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
Q7F19_RS31210 (Q7F19_31210)	pilT	6633693..6634727 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
Q7F19_RS31215 (Q7F19_31215)	-	6634942..6635634 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
Q7F19_RS31220 (Q7F19_31220)	proC	6635646..6636467 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
Q7F19_RS31225 (Q7F19_31225)	-	6636478..6637071 (+)	594	WP_003084544.1	YggT family protein	-
Q7F19_RS31230 (Q7F19_31230)	-	6637068..6637364 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=862246 Q7F19_RS31205 WP_003110016.1 6632367..6633515(-) (pilU) [Pseudomonas aeruginosa strain 2023CK-00048]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=862246 Q7F19_RS31205 WP_003110016.1 6632367..6633515(-) (pilU) [Pseudomonas aeruginosa strain 2023CK-00048]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382