Detailed information NTG0862204

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: WCU05_RS02455
• Genome accession: NZ_CP147622
• Coordinates: 522924..524072 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain GN06251
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 517924..529072

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WCU05_RS02430 (WCU05_02430)	-	519075..519371 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
WCU05_RS02435 (WCU05_02435)	-	519368..519961 (-)	594	WP_003084544.1	YggT family protein	-
WCU05_RS02440 (WCU05_02440)	proC	519972..520793 (-)	822	WP_023081631.1	pyrroline-5-carboxylate reductase	-
WCU05_RS02445 (WCU05_02445)	-	520805..521497 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
WCU05_RS02450 (WCU05_02450)	pilT	521712..522746 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
WCU05_RS02455 (WCU05_02455)	pilU	522924..524072 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
WCU05_RS02460 (WCU05_02460)	czcD	524080..524979 (-)	900	WP_023081630.1	CDF family zinc transporter CzcD	-
WCU05_RS02465 (WCU05_02465)	-	525133..525537 (+)	405	WP_003084560.1	NINE protein	-
WCU05_RS02470 (WCU05_02470)	-	525780..527153 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
WCU05_RS02475 (WCU05_02475)	-	527150..528334 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=862204 WCU05_RS02455 WP_003100959.1 522924..524072(+) (pilU) [Pseudomonas aeruginosa strain GN06251]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=862204 WCU05_RS02455 WP_003100959.1 522924..524072(+) (pilU) [Pseudomonas aeruginosa strain GN06251]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382