Detailed information NTG0861362

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: Q5420_RS02040
• Genome accession: NZ_CP130751
• Coordinates: 442758..443906 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain AU10241 isolate AU10241_L13_Control
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 437758..448906

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q5420_RS02015 (Q5420_02015)	-	438909..439205 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
Q5420_RS02020 (Q5420_02020)	-	439202..439795 (-)	594	WP_003084544.1	YggT family protein	-
Q5420_RS02025 (Q5420_02025)	proC	439806..440627 (-)	822	WP_061190253.1	pyrroline-5-carboxylate reductase	-
Q5420_RS02030 (Q5420_02030)	-	440639..441331 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
Q5420_RS02035 (Q5420_02035)	pilT	441546..442580 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
Q5420_RS02040 (Q5420_02040)	pilU	442758..443906 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
Q5420_RS02045 (Q5420_02045)	czcD	443914..444813 (-)	900	WP_061190254.1	CDF family zinc transporter CzcD	-
Q5420_RS02050 (Q5420_02050)	-	444967..445371 (+)	405	WP_003084560.1	NINE protein	-
Q5420_RS02055 (Q5420_02055)	-	445614..446987 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
Q5420_RS02060 (Q5420_02060)	-	446984..448169 (+)	1186	Protein_410	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=861362 Q5420_RS02040 WP_003100959.1 442758..443906(+) (pilU) [Pseudomonas aeruginosa strain AU10241 isolate AU10241_L13_Control]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=861362 Q5420_RS02040 WP_003100959.1 442758..443906(+) (pilU) [Pseudomonas aeruginosa strain AU10241 isolate AU10241_L13_Control]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGTGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382