Detailed information NTG0860844

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: K9C73_RS14820
• Genome accession: NZ_CP130628
• Coordinates: 3085110..3086228 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain MRSN576822
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3080110..3091228

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K9C73_RS14810 (K9C73_11415)	-	3083226..3083918 (-)	693	WP_001108506.1	YggS family pyridoxal phosphate-dependent enzyme	-
K9C73_RS14815 (K9C73_11410)	pilT	3084045..3085082 (+)	1038	WP_000355491.1	type IV pilus twitching motility protein PilT	Machinery gene
K9C73_RS14820 (K9C73_11405)	pilU	3085110..3086228 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
K9C73_RS14825 (K9C73_11400)	fur	3086298..3086735 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
K9C73_RS14830 (K9C73_11395)	-	3086847..3087245 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
K9C73_RS14835 (K9C73_11390)	-	3087300..3087623 (-)	324	WP_000442585.1	RnfH family protein	-
K9C73_RS14840 (K9C73_11385)	-	3087623..3088690 (-)	1068	WP_000092403.1	hypothetical protein	-
K9C73_RS14845 (K9C73_11380)	-	3088843..3089298 (-)	456	WP_000782976.1	bacteriohemerythrin	-
K9C73_RS14850 (K9C73_11375)	-	3089551..3090399 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=860844 K9C73_RS14820 WP_000347036.1 3085110..3086228(+) (pilU) [Acinetobacter baumannii strain MRSN576822]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=860844 K9C73_RS14820 WP_000347036.1 3085110..3086228(+) (pilU) [Acinetobacter baumannii strain MRSN576822]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGTGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392