Detailed information NTG0860798

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: K9C72_RS14535
• Genome accession: NZ_CP130627
• Coordinates: 3047770..3048888 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain MRSN571146
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3042770..3053888

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K9C72_RS14525 (K9C72_07190)	-	3045886..3046578 (-)	693	WP_001108506.1	YggS family pyridoxal phosphate-dependent enzyme	-
K9C72_RS14530 (K9C72_07195)	pilT	3046705..3047742 (+)	1038	WP_000355491.1	type IV pilus twitching motility protein PilT	Machinery gene
K9C72_RS14535 (K9C72_07200)	pilU	3047770..3048888 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
K9C72_RS14540 (K9C72_07205)	fur	3048958..3049395 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
K9C72_RS14545 (K9C72_07210)	-	3049507..3049905 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
K9C72_RS14550 (K9C72_07215)	-	3049960..3050283 (-)	324	WP_000442585.1	RnfH family protein	-
K9C72_RS14555 (K9C72_07220)	-	3050283..3051350 (-)	1068	WP_000092403.1	hypothetical protein	-
K9C72_RS14560 (K9C72_07225)	-	3051503..3051958 (-)	456	WP_000782976.1	bacteriohemerythrin	-
K9C72_RS14565 (K9C72_07230)	-	3052211..3053059 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=860798 K9C72_RS14535 WP_000347036.1 3047770..3048888(+) (pilU) [Acinetobacter baumannii strain MRSN571146]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=860798 K9C72_RS14535 WP_000347036.1 3047770..3048888(+) (pilU) [Acinetobacter baumannii strain MRSN571146]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGTGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392