Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   LYSHEL_RS08795 Genome accession   NZ_AP024546
Coordinates   1802564..1803622 (+) Length   352 a.a.
NCBI ID   WP_213433560.1    Uniprot ID   -
Organism   Lysobacter helvus strain JCM32364     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1797564..1808622
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LYSHEL_RS08780 (LYSHEL_17290) - 1797770..1799059 (+) 1290 WP_213433555.1 citrate synthase -
  LYSHEL_RS08785 (LYSHEL_17300) - 1799123..1799770 (-) 648 WP_213433556.1 hypothetical protein -
  LYSHEL_RS08790 (LYSHEL_17310) - 1799832..1802330 (-) 2499 WP_213433558.1 penicillin-binding protein 1A -
  LYSHEL_RS08795 (LYSHEL_17320) pilM 1802564..1803622 (+) 1059 WP_213433560.1 pilus assembly protein PilM Machinery gene
  LYSHEL_RS08800 (LYSHEL_17330) - 1803622..1804353 (+) 732 WP_213433562.1 PilN domain-containing protein -
  LYSHEL_RS08805 (LYSHEL_17340) pilO 1804350..1805021 (+) 672 WP_213433564.1 type 4a pilus biogenesis protein PilO -
  LYSHEL_RS08810 (LYSHEL_17350) - 1805023..1805556 (+) 534 WP_213433566.1 pilus assembly protein PilP -
  LYSHEL_RS08815 (LYSHEL_17360) - 1805568..1807529 (+) 1962 WP_213433568.1 type IV pilus secretin PilQ family protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37659.19 Da        Isoelectric Point: 4.3920

>NTDB_id=85860 LYSHEL_RS08795 WP_213433560.1 1802564..1803622(+) (pilM) [Lysobacter helvus strain JCM32364]
MGLITNSQPPLIGVDISSTAVKLLQLSRAGNRYRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIKRAVARSGARARHAAAA
VAGSAVITKIIPMPADLDEDELESQVELEAVNYIPYPIEEVNLDFEVLGAMPGNVEMVQVLLAASRTENVDARVSALELG
GLSARVMDVEAFAIENAFSLLADGLSAPRDGIIAMVDTGATMTTLNILRNGRSLYSREQVFGGKQLTDEVMRRYGLSYEE
AGLAKRQGGLPESYEIEVLEPFKEAMVQQVSRLLQFFYAGSEFNRVDQIVLAGGCASIPGIAAMVEEQLGVPTVVANPLA
QMTLGPRVQAHALAQDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=85860 LYSHEL_RS08795 WP_213433560.1 1802564..1803622(+) (pilM) [Lysobacter helvus strain JCM32364]
GTGGGTCTGATCACAAATAGTCAGCCGCCCTTGATCGGCGTCGACATCAGTTCGACTGCGGTCAAGTTGCTGCAGCTCTC
CCGTGCCGGGAACCGCTATCGCGTAGAGCACTACGCGGTCGAGCCGCTGCCGCCGAACGCCGTCGTCGAGAAGAACATCG
TCGAAGTCGAGGCGGTGGGCGAAGCGATCAAGCGTGCCGTGGCACGGTCCGGCGCCCGCGCGCGCCATGCGGCCGCGGCC
GTCGCCGGGTCGGCGGTGATCACCAAGATCATCCCGATGCCCGCCGACCTGGACGAGGACGAACTGGAATCCCAGGTCGA
ACTCGAAGCGGTCAATTACATCCCGTACCCGATCGAGGAAGTGAACCTCGACTTCGAGGTGCTGGGCGCCATGCCGGGCA
ACGTCGAAATGGTGCAGGTGCTGCTCGCGGCCTCGCGCACCGAGAACGTCGATGCCCGCGTGTCCGCGCTGGAACTCGGC
GGCCTCTCGGCCCGGGTGATGGACGTGGAAGCCTTCGCCATCGAGAACGCGTTCTCGCTGCTCGCCGACGGCCTGTCCGC
GCCGCGCGACGGGATCATCGCGATGGTCGACACCGGCGCCACGATGACCACGCTCAACATCCTCCGCAACGGTCGCAGCC
TCTACAGCCGCGAACAGGTGTTCGGCGGCAAGCAGCTCACCGACGAAGTGATGCGCCGCTACGGCCTGAGCTACGAGGAA
GCCGGCCTGGCCAAGCGCCAGGGCGGGCTGCCGGAAAGCTACGAGATCGAAGTGCTCGAGCCCTTCAAGGAAGCGATGGT
CCAGCAGGTCAGCCGACTGTTGCAGTTCTTCTACGCGGGCAGCGAATTCAACCGTGTCGACCAGATCGTCCTCGCCGGCG
GTTGCGCCTCCATCCCCGGCATCGCCGCGATGGTGGAAGAACAGCTCGGCGTGCCGACCGTGGTGGCCAACCCGCTCGCG
CAAATGACGCTCGGCCCGCGCGTGCAGGCACACGCCCTCGCGCAGGACGCCCCCGCGCTGATGATCGCCTGCGGCCTCGC
CCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

53.509

97.159

0.52
  comM Acinetobacter nosocomialis M2

53.216

97.159

0.517
  comM Acinetobacter baylyi ADP1

51.754

97.159

0.503
  pilM Legionella pneumophila strain ERS1305867

44.759

100

0.449

Multiple sequence alignment