Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LYSHEL_RS07900 Genome accession   NZ_AP024546
Coordinates   1612031..1613284 (-) Length   417 a.a.
NCBI ID   WP_213437397.1    Uniprot ID   -
Organism   Lysobacter helvus strain JCM32364     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1607031..1618284
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LYSHEL_RS07865 (LYSHEL_15510) - 1607361..1608488 (+) 1128 WP_213437385.1 sensor domain-containing diguanylate cyclase -
  LYSHEL_RS07870 (LYSHEL_15520) - 1608575..1609528 (+) 954 WP_213437387.1 ABC transporter ATP-binding protein -
  LYSHEL_RS07875 (LYSHEL_15530) - 1609525..1610286 (+) 762 WP_213437389.1 ABC transporter permease -
  LYSHEL_RS07880 (LYSHEL_15540) - 1610527..1610937 (-) 411 WP_213437391.1 VOC family protein -
  LYSHEL_RS07885 (LYSHEL_15550) - 1610970..1611410 (-) 441 WP_244858706.1 helix-turn-helix transcriptional regulator -
  LYSHEL_RS07890 (LYSHEL_15560) - 1611407..1611787 (-) 381 WP_213437393.1 YciI family protein -
  LYSHEL_RS07895 (LYSHEL_15570) - 1611872..1612030 (+) 159 WP_213437395.1 hypothetical protein -
  LYSHEL_RS07900 (LYSHEL_15580) pilU 1612031..1613284 (-) 1254 WP_213437397.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LYSHEL_RS07905 (LYSHEL_15590) - 1613393..1613656 (-) 264 WP_213437399.1 DUF3297 family protein -
  LYSHEL_RS07910 (LYSHEL_15600) - 1613695..1613979 (-) 285 WP_213437401.1 DUF4031 domain-containing protein -
  LYSHEL_RS07915 (LYSHEL_15610) - 1614037..1615968 (+) 1932 WP_213437403.1 ABC transporter ATP-binding protein/permease -
  LYSHEL_RS07920 (LYSHEL_15620) - 1615970..1616776 (+) 807 WP_213437405.1 NRDE family protein -
  LYSHEL_RS07925 (LYSHEL_15630) - 1616871..1617173 (+) 303 WP_213437407.1 carboxypeptidase-like regulatory domain-containing protein -

Sequence

Protein


Download         Length: 417 a.a.        Molecular weight: 46490.29 Da        Isoelectric Point: 7.0468

>NTDB_id=85857 LYSHEL_RS07900 WP_213437397.1 1612031..1613284(-) (pilU) [Lysobacter helvus strain JCM32364]
MLSDEQAKDYMHKLLAAMAKAGGSDLFIAHDYPPSMKAHGSMTPLTQQKLTSEVTASLARCLMNEKQRAEFAEQMECNFA
IVVPGVSRFRVNVFVQQGNVGMVLRTIAAEIPNFEKLRLPEVLKDVVMEKRGLVLLVGGTGSGKSTSLAAMIDHRNRTSA
GHIITVEDPVEYAHVSQKSLVTHREVGVDTHSWHHALKNTLRQAPDVILVGEIRDAETMEHAISFAETGHLCLSTLHANS
ASQTMERIINFFPEERRTQLLMDLSANLRAIVSQRLVRTEDGKGRVAAIEILLNTPTIAEKIFKGEFNELKGVMTKSREL
GMRTFDWALFELYNDGLISYEEAIRNADSANELRLNIKLKSKRGEPSNAAGTSLELHHQPTPAELEAMRQEELRKQQFKR
DEIELQRLMAAKQAAAG

Nucleotide


Download         Length: 1254 bp        

>NTDB_id=85857 LYSHEL_RS07900 WP_213437397.1 1612031..1613284(-) (pilU) [Lysobacter helvus strain JCM32364]
ATGTTGAGCGACGAACAAGCCAAGGACTACATGCACAAGCTGCTGGCGGCGATGGCCAAGGCCGGCGGCTCCGACTTGTT
CATCGCGCACGACTACCCGCCGAGCATGAAGGCGCACGGGTCGATGACGCCGCTCACGCAGCAGAAGCTCACCAGCGAAG
TCACCGCGTCCCTCGCCCGCTGCCTGATGAACGAGAAGCAGCGCGCCGAGTTCGCCGAGCAGATGGAGTGCAACTTCGCC
ATCGTGGTGCCCGGCGTGTCGCGCTTCCGCGTCAACGTGTTCGTGCAGCAGGGCAACGTGGGCATGGTGCTGCGCACGAT
CGCCGCGGAAATCCCGAATTTCGAAAAGCTGCGCCTCCCCGAGGTCCTGAAGGACGTCGTGATGGAGAAGCGCGGCCTGG
TGCTGCTGGTCGGCGGCACGGGCTCGGGCAAGTCCACGTCGCTGGCGGCGATGATCGACCACCGCAACCGCACCTCGGCC
GGCCACATCATCACGGTCGAGGATCCGGTCGAATACGCGCACGTCTCGCAGAAGTCGCTGGTCACGCACCGCGAAGTCGG
CGTCGACACGCATTCGTGGCACCACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGGTCGGCGAGATCC
GCGACGCGGAAACGATGGAGCACGCGATCTCCTTCGCCGAGACCGGCCACCTGTGCCTGAGCACCCTGCACGCCAACAGC
GCGTCGCAGACGATGGAACGCATCATCAACTTCTTCCCGGAAGAACGCCGCACGCAGCTGCTGATGGACCTGTCGGCCAA
CCTGCGCGCGATCGTCTCGCAGCGCCTGGTGCGCACCGAGGACGGCAAGGGCCGCGTCGCCGCGATCGAAATCCTGCTCA
ACACGCCCACCATCGCCGAGAAGATCTTCAAGGGCGAGTTCAACGAGCTCAAGGGCGTGATGACCAAGTCGCGCGAACTG
GGCATGCGCACGTTCGACTGGGCGTTGTTCGAGCTCTACAACGACGGGCTGATCTCGTACGAAGAAGCCATCCGCAACGC
GGACTCGGCGAACGAGCTGCGCCTGAACATCAAGTTGAAGAGCAAGCGCGGCGAGCCGTCGAACGCCGCCGGGACGTCGC
TGGAGCTCCACCACCAGCCCACGCCGGCGGAACTGGAAGCGATGCGCCAGGAAGAACTGCGCAAGCAGCAGTTCAAGCGC
GACGAGATCGAACTGCAGCGCCTGATGGCGGCCAAGCAGGCCGCGGCGGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.038

88.01

0.511
  pilU Acinetobacter baylyi ADP1

52.542

84.892

0.446
  pilU Vibrio cholerae strain A1552

50.141

85.132

0.427
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.464

93.046

0.376

Multiple sequence alignment