Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LYSCAS_RS07900 Genome accession   NZ_AP024545
Coordinates   1612043..1613266 (-) Length   407 a.a.
NCBI ID   WP_407075179.1    Uniprot ID   -
Organism   Noviluteimonas caseinilytica strain JCM32879     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1607043..1618266
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LYSCAS_RS07865 (LYSCAS_15510) - 1607373..1608500 (+) 1128 WP_213437385.1 sensor domain-containing diguanylate cyclase -
  LYSCAS_RS07870 (LYSCAS_15520) - 1608587..1609540 (+) 954 WP_213437387.1 ABC transporter ATP-binding protein -
  LYSCAS_RS07875 (LYSCAS_15530) - 1609537..1610298 (+) 762 WP_213437389.1 ABC transporter permease -
  LYSCAS_RS07880 (LYSCAS_15540) - 1610539..1610949 (-) 411 WP_213437391.1 VOC family protein -
  LYSCAS_RS07885 (LYSCAS_15550) - 1610982..1611422 (-) 441 WP_244858706.1 helix-turn-helix transcriptional regulator -
  LYSCAS_RS07890 (LYSCAS_15560) - 1611419..1611799 (-) 381 WP_213437393.1 YciI family protein -
  LYSCAS_RS07895 (LYSCAS_15570) - 1611884..1612042 (+) 159 WP_213437395.1 hypothetical protein -
  LYSCAS_RS07900 (LYSCAS_15580) pilU 1612043..1613266 (-) 1224 WP_407075179.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LYSCAS_RS07905 (LYSCAS_15590) - 1613405..1613668 (-) 264 WP_213437399.1 DUF3297 family protein -
  LYSCAS_RS07910 (LYSCAS_15600) - 1613707..1613991 (-) 285 WP_213437401.1 DUF4031 domain-containing protein -
  LYSCAS_RS07915 (LYSCAS_15610) - 1614049..1615980 (+) 1932 WP_213437403.1 ABC transporter ATP-binding protein/permease -
  LYSCAS_RS07920 (LYSCAS_15620) - 1615982..1616788 (+) 807 WP_213437405.1 NRDE family protein -
  LYSCAS_RS07925 (LYSCAS_15630) - 1616883..1617185 (+) 303 WP_213437407.1 carboxypeptidase-like regulatory domain-containing protein -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45309.01 Da        Isoelectric Point: 7.4043

>NTDB_id=85838 LYSCAS_RS07900 WP_407075179.1 1612043..1613266(-) (pilU) [Noviluteimonas caseinilytica strain JCM32879]
MHKLLAAMAKAGGSDLFIAHDYPPSMKAHGSMTPLTQQKLTSEVTASLARCLMNEKQRAEFAEQMECNFAIVVPGVSRFR
VNVFVQQGNVGMVLRTIAAEIPNFEKLRLPEVLKDVVMEKRGLVLLVGGTGSGKSTSLAAMIDHRNRTSAGHIITVEDPV
EYAHVSQKSLVTHREVGVDTHSWHHALKNTLRQAPDVILVGEIRDAETMEHAISFAETGHLCLSTLHANSASQTMERIIN
FFPEERRTQLLMDLSANLRAIVSQRLVRTEDGKGRVAAIEILLNTPTIAEKIFKGEFNELKGVMTKSRELGMRTFDWALF
ELYNDGLISYEEAIRNADSANELRLNIKLKSKRGEPSNAAGTSLELHHQPTPAELEAMRQEELRKQQFKRDEIELQRLMA
AKQAAAG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=85838 LYSCAS_RS07900 WP_407075179.1 1612043..1613266(-) (pilU) [Noviluteimonas caseinilytica strain JCM32879]
ATGCACAAGCTGCTGGCGGCGATGGCCAAGGCCGGCGGCTCCGACTTGTTCATCGCGCACGACTACCCGCCGAGCATGAA
GGCGCACGGGTCGATGACGCCGCTCACGCAGCAGAAGCTCACCAGCGAAGTCACCGCGTCCCTCGCCCGCTGCCTGATGA
ACGAGAAGCAGCGCGCCGAGTTCGCCGAGCAGATGGAGTGCAACTTCGCCATCGTGGTGCCCGGCGTGTCGCGCTTCCGC
GTCAACGTGTTCGTGCAGCAGGGCAACGTGGGCATGGTGCTGCGCACGATCGCCGCGGAAATCCCGAATTTCGAAAAGCT
GCGCCTCCCCGAGGTCCTGAAGGACGTCGTGATGGAGAAGCGCGGCCTGGTGCTGCTGGTCGGCGGCACGGGCTCGGGCA
AGTCCACGTCGCTGGCGGCGATGATCGACCACCGCAACCGCACCTCGGCCGGCCACATCATCACGGTCGAGGATCCGGTC
GAATACGCGCACGTCTCGCAGAAGTCGCTGGTCACGCACCGCGAAGTCGGCGTCGACACGCATTCGTGGCACCACGCGCT
GAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGGTCGGCGAGATCCGCGACGCGGAAACGATGGAGCACGCGATCT
CCTTCGCCGAGACCGGCCACCTGTGCCTGAGCACCCTGCACGCCAACAGCGCGTCGCAGACGATGGAACGCATCATCAAC
TTCTTCCCGGAAGAACGCCGCACGCAGCTGCTGATGGACCTGTCGGCCAACCTGCGCGCGATCGTCTCGCAGCGCCTGGT
GCGCACCGAGGACGGCAAGGGCCGCGTCGCCGCGATCGAAATCCTGCTCAACACGCCCACCATCGCCGAGAAGATCTTCA
AGGGCGAGTTCAACGAGCTCAAGGGCGTGATGACCAAGTCGCGCGAACTGGGCATGCGCACGTTCGACTGGGCGTTGTTC
GAGCTCTACAACGACGGGCTGATCTCGTACGAAGAAGCCATCCGCAACGCGGACTCGGCGAACGAGCTGCGCCTGAACAT
CAAGTTGAAGAGCAAGCGCGGCGAGCCGTCGAACGCCGCCGGGACGTCGCTGGAGCTCCACCACCAGCCCACGCCGGCGG
AACTGGAAGCGATGCGCCAGGAAGAACTGCGCAAGCAGCAGTTCAAGCGCGACGAGATCGAACTGCAGCGCCTGATGGCG
GCCAAGCAGGCCGCGGCGGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.038

90.172

0.523
  pilU Acinetobacter baylyi ADP1

52.101

87.715

0.457
  pilU Vibrio cholerae strain A1552

50.141

87.224

0.437
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.464

95.332

0.386

Multiple sequence alignment