Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   Q3F87_RS02640 Genome accession   NZ_CP130328
Coordinates   379953..380462 (+) Length   169 a.a.
NCBI ID   WP_302700360.1    Uniprot ID   -
Organism   Acinetobacter variabilis strain TB2-2B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 374265..397441 379953..380462 within 0


Gene organization within MGE regions


Location: 374265..397441
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q3F87_RS02605 (Q3F87_02605) - 374265..374777 (+) 513 WP_157937950.1 hypothetical protein -
  Q3F87_RS02610 (Q3F87_02610) - 374819..375787 (-) 969 WP_302700650.1 IS30 family transposase -
  Q3F87_RS02615 (Q3F87_02615) - 375899..377299 (+) 1401 Protein_327 hypothetical protein -
  Q3F87_RS02620 (Q3F87_02620) - 377303..377659 (+) 357 WP_302700354.1 hypothetical protein -
  Q3F87_RS02625 (Q3F87_02625) - 377698..378177 (-) 480 WP_302700356.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  Q3F87_RS02630 (Q3F87_02630) - 378716..378886 (+) 171 WP_302700358.1 hypothetical protein -
  Q3F87_RS02635 (Q3F87_02635) - 379009..379770 (+) 762 WP_302700359.1 hypothetical protein -
  Q3F87_RS02640 (Q3F87_02640) pilE 379953..380462 (+) 510 WP_302700360.1 pilin Machinery gene
  Q3F87_RS02645 (Q3F87_02645) tfpZ 380504..381253 (+) 750 WP_302700362.1 TfpX/TfpZ family type IV pilin accessory protein -
  Q3F87_RS02650 (Q3F87_02650) - 381345..382979 (+) 1635 WP_302700364.1 PglL family O-oligosaccharyltransferase -
  Q3F87_RS02655 (Q3F87_02655) bfr 383034..383498 (-) 465 WP_175966220.1 bacterioferritin -
  Q3F87_RS02660 (Q3F87_02660) - 383725..383919 (-) 195 WP_004787097.1 bacterioferritin-associated ferredoxin -
  Q3F87_RS02665 (Q3F87_02665) - 384141..384524 (-) 384 WP_302700365.1 RidA family protein -
  Q3F87_RS02670 (Q3F87_02670) - 384656..385714 (+) 1059 WP_302700366.1 transposase -
  Q3F87_RS02675 (Q3F87_02675) - 385791..387896 (-) 2106 WP_180025548.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  Q3F87_RS02680 (Q3F87_02680) rpoZ 388114..388395 (-) 282 WP_004650579.1 DNA-directed RNA polymerase subunit omega -
  Q3F87_RS02685 (Q3F87_02685) gmk 388473..389096 (-) 624 WP_004787090.1 guanylate kinase -
  Q3F87_RS02690 (Q3F87_02690) ispH 389232..390182 (+) 951 WP_180053730.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  Q3F87_RS02695 (Q3F87_02695) - 390345..390776 (+) 432 WP_284879441.1 Tfp pilus assembly protein FimT/FimU -
  Q3F87_RS02700 (Q3F87_02700) pilV 390773..391318 (+) 546 WP_168542238.1 type IV pilus modification protein PilV -
  Q3F87_RS02705 (Q3F87_02705) - 391315..392226 (+) 912 WP_302700367.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  Q3F87_RS02710 (Q3F87_02710) - 392226..393038 (+) 813 WP_302700368.1 PilX N-terminal domain-containing pilus assembly protein -
  Q3F87_RS02715 (Q3F87_02715) - 393053..396496 (+) 3444 WP_302700369.1 pilus assembly protein -
  Q3F87_RS02720 (Q3F87_02720) - 396507..396968 (+) 462 WP_302700370.1 type IV pilin protein -
  Q3F87_RS02725 (Q3F87_02725) - 396956..397441 (+) 486 WP_284879435.1 type IV pilin protein -

Sequence

Protein


Download         Length: 169 a.a.        Molecular weight: 17109.76 Da        Isoelectric Point: 8.2287

>NTDB_id=857880 Q3F87_RS02640 WP_302700360.1 379953..380462(+) (pilE) [Acinetobacter variabilis strain TB2-2B]
MECIELMIVVAIIGILAAVAIPAYQNYTVRAKVTEMIAAGASAKSAVSESYQSNGLTGVKAAATALATEQKAGTIASKYV
SKVVIAPTTGIITVTSATDGDISGLPTDALNKTIILTPYVGTTELATAATGGSIDWVCTSKTKATAAARGFTSAKAGTMP
DKYVPAECK

Nucleotide


Download         Length: 510 bp        

>NTDB_id=857880 Q3F87_RS02640 WP_302700360.1 379953..380462(+) (pilE) [Acinetobacter variabilis strain TB2-2B]
ATGGAATGTATTGAATTAATGATTGTTGTAGCAATTATTGGTATTTTGGCGGCAGTTGCTATTCCGGCTTACCAAAATTA
TACAGTTCGTGCTAAAGTAACCGAGATGATTGCGGCGGGTGCATCTGCTAAATCAGCTGTGAGCGAATCTTATCAAAGTA
ATGGATTAACAGGTGTAAAAGCTGCTGCAACTGCGCTTGCAACTGAACAAAAAGCTGGAACAATAGCTAGTAAATATGTA
AGCAAAGTTGTTATTGCTCCAACTACTGGTATAATTACAGTAACATCTGCAACTGATGGAGATATTTCTGGGTTACCTAC
TGATGCTTTAAATAAAACTATTATTTTAACACCATATGTTGGCACTACCGAATTGGCAACTGCAGCTACTGGTGGTAGTA
TTGACTGGGTATGTACTTCTAAAACTAAGGCGACAGCTGCAGCTCGAGGTTTTACTTCTGCCAAAGCAGGTACTATGCCT
GATAAATATGTTCCAGCTGAATGTAAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(29-169)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

45.856

100

0.491
  pilA Ralstonia pseudosolanacearum GMI1000

39.655

100

0.408
  pilA2 Legionella pneumophila strain ERS1305867

38.922

98.817

0.385
  pilA2 Legionella pneumophila str. Paris

37.725

98.817

0.373