Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   QUE97_RS08010 Genome accession   NZ_AP027432
Coordinates   1096713..1097723 (-) Length   336 a.a.
NCBI ID   WP_000568522.1    Uniprot ID   Q8FGR3
Organism   Escherichia coli strain EC581 isolate EC581     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1091713..1102723
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUE97_RS07990 (MUTS9_15460) mepM 1092679..1094001 (-) 1323 WP_001184045.1 murein DD-endopeptidase MepM -
  QUE97_RS07995 (MUTS9_15470) znuA 1094017..1094949 (-) 933 WP_001376899.1 zinc ABC transporter substrate-binding protein ZnuA -
  QUE97_RS08000 (MUTS9_15480) znuC 1095028..1095783 (+) 756 WP_024226334.1 zinc ABC transporter ATP-binding protein ZnuC -
  QUE97_RS08005 (MUTS9_15490) znuB 1095780..1096565 (+) 786 WP_000571471.1 zinc ABC transporter permease subunit ZnuB -
  QUE97_RS08010 (MUTS9_15500) ruvB 1096713..1097723 (-) 1011 WP_000568522.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  QUE97_RS08015 (MUTS9_15510) ruvA 1097732..1098343 (-) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  QUE97_RS08020 yobI 1098482..1098547 (-) 66 WP_072093883.1 stress response small protein YobI -
  QUE97_RS08025 (MUTS9_15520) yebB 1098618..1099220 (+) 603 WP_001024929.1 YebB family permuted papain-like enzyme -
  QUE97_RS08030 (MUTS9_15530) ruvC 1099222..1099743 (-) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  QUE97_RS08035 (MUTS9_15540) yebC 1099778..1100518 (-) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  QUE97_RS08040 (MUTS9_15550) nudB 1100547..1100999 (-) 453 WP_032141314.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37171.80 Da        Isoelectric Point: 4.7818

>NTDB_id=85787 QUE97_RS08010 WP_000568522.1 1096713..1097723(-) (ruvB) [Escherichia coli strain EC581 isolate EC581]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TVRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=85787 QUE97_RS08010 WP_000568522.1 1096713..1097723(-) (ruvB) [Escherichia coli strain EC581 isolate EC581]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCGCAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCTAACATTGTCGCCAATGAAATGGGT
GTTAATTTACGTACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTCTTTCGCCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAGCGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCACGTATTGCCAACCGTCTGCTGCGTCGAGTGCGTGACTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCTGATATCGCTGCTCAGGCGCTGGATATGCTGAATGTCGATGCCGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCTGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGCGGGCGTATGGCG
ACAGTGCGGGCGTGGAACCACTTCGGCATCACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8FGR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

52.941

96.131

0.509
  ruvB Helicobacter pylori 26695

52.366

94.345

0.494

Multiple sequence alignment