Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QYS58_RS01240 Genome accession   NZ_CP130143
Coordinates   263834..264976 (+) Length   380 a.a.
NCBI ID   WP_302140337.1    Uniprot ID   -
Organism   Halomonas alkalicola strain M2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 258834..269976
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QYS58_RS01215 - 259223..260380 (-) 1158 WP_110070044.1 homoserine O-acetyltransferase -
  QYS58_RS01220 - 260427..261008 (-) 582 WP_169955853.1 YggT family protein -
  QYS58_RS01225 proC 261071..261895 (-) 825 WP_302140334.1 pyrroline-5-carboxylate reductase -
  QYS58_RS01230 - 261966..262685 (-) 720 WP_119022736.1 YggS family pyridoxal phosphate-dependent enzyme -
  QYS58_RS01235 pilT 262752..263786 (+) 1035 WP_119022735.1 type IV pilus twitching motility protein PilT Machinery gene
  QYS58_RS01240 pilU 263834..264976 (+) 1143 WP_302140337.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QYS58_RS01245 - 265017..266156 (-) 1140 WP_169955849.1 anhydro-N-acetylmuramic acid kinase -
  QYS58_RS01250 tyrS 266272..267471 (+) 1200 WP_169955847.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42215.53 Da        Isoelectric Point: 6.1385

>NTDB_id=857316 QYS58_RS01240 WP_302140337.1 263834..264976(+) (pilU) [Halomonas alkalicola strain M2]
MTAKEWLYQLLDIMVQKEASDLLISVNAPPTLKMAGKLTPLGDQGLSVAQVDELVSASIPEAVMARFQVEREANFALSLR
DKGRFRVSAFQQRNQKAMVIRRIAFEIPHLEELSLPPVLGELANNKRGLVFVVGGTGTGKSTTLASMIQQRNETLGGHII
SVEDPIEYVHPHKKAIINQREVGIDTDSFEVALKNTLRQAPDVILIGEIRTRETMEHALTFAETGHLCLATLHANNANQA
LDRIIHFFPHERHEQIWLDLSLNMRAIVAQQLLPTVDGGRCPAIEIMLKSPLIGDLVRKGEVNEIKNVMSRSRDLGMQTF
DQALYDLFRAGKITEEVALVHADSANDLRMMIKYGDAGSEGLAQAREAASQLSLKRDEDF

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=857316 QYS58_RS01240 WP_302140337.1 263834..264976(+) (pilU) [Halomonas alkalicola strain M2]
ATGACGGCGAAAGAGTGGCTCTACCAGCTGCTGGATATCATGGTGCAGAAGGAGGCGTCGGATCTGCTGATCTCGGTGAA
TGCACCGCCGACCCTCAAGATGGCCGGCAAGCTGACTCCCCTGGGCGACCAGGGGCTCTCCGTGGCCCAGGTCGACGAGC
TGGTCAGCGCGTCGATCCCGGAGGCGGTGATGGCGCGCTTCCAGGTCGAGCGCGAGGCCAACTTCGCGCTCAGCCTCAGG
GACAAGGGGCGCTTTCGCGTCAGCGCCTTCCAGCAGCGCAACCAGAAGGCGATGGTGATCCGGCGCATCGCCTTCGAGAT
CCCCCACCTGGAGGAGCTGTCGCTGCCGCCGGTGCTGGGCGAGCTGGCCAACAACAAGCGTGGCCTGGTGTTCGTGGTGG
GCGGCACCGGTACCGGCAAGTCGACCACCCTGGCCTCGATGATCCAGCAGCGCAACGAGACCCTGGGGGGCCATATCATC
AGCGTCGAGGATCCCATCGAGTACGTGCATCCGCACAAGAAGGCGATCATCAACCAGCGCGAGGTGGGCATCGACACCGA
CTCCTTCGAGGTCGCGCTGAAGAACACCCTGCGCCAGGCGCCCGACGTGATCCTGATCGGCGAGATCCGCACCCGGGAGA
CCATGGAGCATGCGCTGACCTTCGCCGAGACCGGCCACCTCTGCCTGGCGACCCTGCACGCCAACAACGCCAACCAGGCG
CTGGATCGCATCATCCACTTCTTCCCCCACGAGCGGCACGAGCAGATCTGGCTCGACCTCTCGCTCAACATGCGCGCCAT
CGTGGCCCAGCAGCTGCTGCCCACGGTGGATGGTGGCCGCTGCCCGGCCATCGAGATCATGCTCAAGTCACCGCTGATCG
GCGATCTGGTACGCAAGGGCGAGGTCAACGAGATCAAGAACGTGATGTCGCGCTCCCGAGACCTGGGGATGCAGACCTTC
GACCAGGCGCTCTACGACCTCTTCCGGGCGGGCAAGATCACCGAGGAGGTGGCGCTGGTGCACGCCGACTCGGCCAACGA
CCTGCGCATGATGATCAAGTACGGTGACGCCGGCAGCGAAGGGCTGGCCCAGGCACGCGAGGCGGCAAGCCAGCTCTCGC
TCAAGCGCGACGAGGACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(66-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.59

98.158

0.595
  pilU Vibrio cholerae strain A1552

60.057

91.579

0.55
  pilU Acinetobacter baylyi ADP1

54.62

96.842

0.529
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.159

100

0.432