Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   QUF17_RS07635 Genome accession   NZ_AP027417
Coordinates   883389..884399 (-) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain EC361 isolate EC361     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 878389..889399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUF17_RS07615 (MUTS5_14750) mepM 879356..880678 (-) 1323 WP_001184045.1 murein DD-endopeptidase MepM -
  QUF17_RS07620 (MUTS5_14760) znuA 880694..881626 (-) 933 WP_001342995.1 zinc ABC transporter substrate-binding protein ZnuA -
  QUF17_RS07625 (MUTS5_14770) znuC 881705..882460 (+) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  QUF17_RS07630 (MUTS5_14780) znuB 882457..883242 (+) 786 WP_000571465.1 zinc ABC transporter permease subunit ZnuB -
  QUF17_RS07635 (MUTS5_14790) ruvB 883389..884399 (-) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  QUF17_RS07640 (MUTS5_14800) ruvA 884408..885019 (-) 612 WP_000580328.1 Holliday junction branch migration protein RuvA -
  QUF17_RS07645 yobI 885158..885223 (-) 66 WP_072094247.1 stress response small protein YobI -
  QUF17_RS07650 (MUTS5_14810) yebB 885295..885897 (+) 603 WP_001024929.1 YebB family permuted papain-like enzyme -
  QUF17_RS07655 (MUTS5_14820) ruvC 885899..886420 (-) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  QUF17_RS07660 (MUTS5_14830) yebC 886455..887195 (-) 741 WP_000907248.1 YebC/PmpR family DNA-binding transcriptional regulator -
  QUF17_RS07665 (MUTS5_14840) nudB 887224..887676 (-) 453 WP_001300367.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=85464 QUF17_RS07635 WP_000568519.1 883389..884399(-) (ruvB) [Escherichia coli strain EC361 isolate EC361]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=85464 QUF17_RS07635 WP_000568519.1 883389..884399(-) (ruvB) [Escherichia coli strain EC361 isolate EC361]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTAGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTCGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGTTGTTTATTGATGAGATCCACCGTCTTTCGCCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAGCGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGAGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGTTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
GTTATTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494

Multiple sequence alignment