Detailed information NTG0852106

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: QYH60_RS09915
• Genome accession: NZ_CP129292
• Coordinates: 1986316..1987638 (+)
• Length: 440 a.a.
• NCBI ID: WP_058208412.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain KMGR2-43
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1981316..1992638

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QYH60_RS09890 (QYH60_09890)	glyQ	1981832..1982785 (-)	954	WP_003130667.1	glycine--tRNA ligase subunit alpha	-
QYH60_RS09895 (QYH60_09895)	-	1983070..1983816 (-)	747	WP_058208415.1	bifunctional metallophosphatase/5'-nucleotidase	-
QYH60_RS09900 (QYH60_09900)	-	1983932..1984951 (-)	1020	WP_058208414.1	DUF475 domain-containing protein	-
QYH60_RS09905 (QYH60_09905)	-	1984999..1985496 (-)	498	WP_058208413.1	VanZ family protein	-
QYH60_RS09910 (QYH60_09910)	-	1985630..1986259 (-)	630	WP_003130660.1	YigZ family protein	-
QYH60_RS09915 (QYH60_09915)	comFA	1986316..1987638 (+)	1323	WP_058208412.1	DEAD/DEAH box helicase	Machinery gene
QYH60_RS09920 (QYH60_09920)	comFC	1987881..1988285 (+)	405	WP_021722514.1	ComF family protein	Machinery gene
QYH60_RS09925 (QYH60_09925)	-	1988319..1988897 (-)	579	WP_301400880.1	nucleotidyltransferase family protein	-
QYH60_RS09930 (QYH60_09930)	-	1988911..1989366 (-)	456	WP_003130653.1	diacylglycerol kinase	-
QYH60_RS09935 (QYH60_09935)	ybeY	1989350..1989838 (-)	489	WP_003130652.1	rRNA maturation RNase YbeY	-
QYH60_RS09940 (QYH60_09940)	-	1989983..1990468 (-)	486	WP_058208410.1	NUDIX hydrolase	-
QYH60_RS09945 (QYH60_09945)	-	1990526..1991494 (-)	969	WP_003130649.1	PhoH family protein	-

Sequence

Protein

• Length: 440 a.a.
• Molecular weight: 50191.77 Da
• Isoelectric Point: 10.1932

>NTDB_id=852106 QYH60_RS09915 WP_058208412.1 1986316..1987638(+) (comFA) [Lactococcus lactis subsp. lactis strain KMGR2-43]
MSTNQEKLFGRLLLKNDILQLIKSTDKISVSKIFSNFLLEAKVNPILGMTSISSNKIKCNRCGTVHIKNSVKLPIGVFYC
PSCIQLGRVRSDEFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDSYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNKMLYF
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKIASVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide

• Length: 1323 bp

>NTDB_id=852106 QYH60_RS09915 WP_058208412.1 1986316..1987638(+) (comFA) [Lactococcus lactis subsp. lactis strain KMGR2-43]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTACAACTTATAAAAAGTACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAGCGAAGGTGAATCCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGTAACCGTTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCGGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTCTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAGC
GGTGGAGTGGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAGCTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATAAAATGCTTTATTTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTTCCTTTAGTCGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTGCTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACTTCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (114-233)

• Helicase conserved C-terminal domain (Helicase_C) (300-403)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	74.75	90.909	0.68
comFA/cflA	Streptococcus mitis SK321	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae Rx1	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae D39	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae R6	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae TIGR4	52.01	90.455	0.47
comFA/cflA	Streptococcus mitis NCTC 12261	51.256	90.455	0.464
comFA	Bacillus subtilis subsp. subtilis str. 168	37.251	100	0.382