Detailed information NTG0085116

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: FLY89_RS04235
• Genome accession: NZ_AP024415
• Coordinates: 893005..894123 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain NIPH17_0019
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 888005..899123

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FLY89_RS04205 (KHAB170019_08080)	-	888834..889682 (+)	849	WP_000336548.1	class II glutamine amidotransferase	-
FLY89_RS04210 (KHAB170019_08090)	-	889935..890390 (+)	456	WP_000782976.1	bacteriohemerythrin	-
FLY89_RS04215 (KHAB170019_08100)	-	890543..891610 (+)	1068	WP_002019572.1	hypothetical protein	-
FLY89_RS04220 (KHAB170019_08110)	-	891610..891933 (+)	324	WP_000442587.1	RnfH family protein	-
FLY89_RS04225 (KHAB170019_08120)	-	891988..892386 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
FLY89_RS04230 (KHAB170019_08130)	fur	892498..892935 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
FLY89_RS04235 (KHAB170019_08140)	pilU	893005..894123 (-)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
FLY89_RS04240 (KHAB170019_08150)	pilT	894151..895188 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
FLY89_RS04245 (KHAB170019_08160)	-	895315..896007 (+)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=85116 FLY89_RS04235 WP_000347039.1 893005..894123(-) (pilU) [Acinetobacter baumannii strain NIPH17_0019]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=85116 FLY89_RS04235 WP_000347039.1 893005..894123(-) (pilU) [Acinetobacter baumannii strain NIPH17_0019]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395