Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   QTV59_RS04220 Genome accession   NZ_CP128526
Coordinates   811622..812101 (+) Length   159 a.a.
NCBI ID   WP_006248401.1    Uniprot ID   A0A249A0S4
Organism   Mannheimia haemolytica strain 171117     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 806622..817101
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QTV59_RS04195 - 807203..807967 (-) 765 WP_006248395.1 TSUP family transporter -
  QTV59_RS04200 proB 807967..809051 (-) 1085 Protein_804 glutamate 5-kinase -
  QTV59_RS04205 folA 809163..809657 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  QTV59_RS04210 - 809777..810007 (+) 231 WP_006248398.1 YgjV family protein -
  QTV59_RS04215 radA 810062..811441 (-) 1380 WP_006248399.1 DNA repair protein RadA -
  QTV59_RS04220 pilA 811622..812101 (+) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  QTV59_RS04225 - 812122..812565 (+) 444 Protein_809 pilus assembly protein PilB -
  QTV59_RS04230 pilC 812571..813767 (+) 1197 WP_006248403.1 type II secretion system F family protein Machinery gene
  QTV59_RS04235 - 813770..814426 (+) 657 WP_006248404.1 prepilin peptidase -
  QTV59_RS04240 coaE 814462..815097 (+) 636 WP_006248405.1 dephospho-CoA kinase -
  QTV59_RS04245 yacG 815079..815264 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  QTV59_RS04250 holA 815265..816290 (-) 1026 WP_006253426.1 DNA polymerase III subunit delta -
  QTV59_RS04255 lptE 816333..816821 (-) 489 WP_006248408.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16841.15 Da        Isoelectric Point: 8.7964

>NTDB_id=848403 QTV59_RS04220 WP_006248401.1 811622..812101(+) (pilA) [Mannheimia haemolytica strain 171117]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=848403 QTV59_RS04220 WP_006248401.1 811622..812101(+) (pilA) [Mannheimia haemolytica strain 171117]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(48-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0S4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

56.429

88.05

0.497
  pilA Haemophilus influenzae Rd KW20

54.93

89.308

0.491