Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   QTV52_RS09265 Genome accession   NZ_CP128525
Coordinates   1752386..1752865 (-) Length   159 a.a.
NCBI ID   WP_006248401.1    Uniprot ID   A0A249A0S4
Organism   Mannheimia haemolytica strain 171555     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1747386..1757865
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QTV52_RS09230 lptE 1747666..1748154 (+) 489 WP_006248408.1 LPS assembly lipoprotein LptE -
  QTV52_RS09235 holA 1748197..1749222 (+) 1026 WP_006253426.1 DNA polymerase III subunit delta -
  QTV52_RS09240 yacG 1749223..1749408 (-) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  QTV52_RS09245 coaE 1749390..1750025 (-) 636 WP_006248405.1 dephospho-CoA kinase -
  QTV52_RS09250 - 1750061..1750717 (-) 657 WP_006248404.1 prepilin peptidase -
  QTV52_RS09255 pilC 1750720..1751916 (-) 1197 WP_006248403.1 type II secretion system F family protein Machinery gene
  QTV52_RS09260 - 1751922..1752365 (-) 444 Protein_1786 pilus assembly protein PilB -
  QTV52_RS09265 pilA 1752386..1752865 (-) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  QTV52_RS09270 radA 1753046..1754425 (+) 1380 WP_006248399.1 DNA repair protein RadA -
  QTV52_RS09275 - 1754480..1754710 (-) 231 WP_006248398.1 YgjV family protein -
  QTV52_RS09280 folA 1754830..1755324 (-) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  QTV52_RS09285 proB 1755436..1756520 (+) 1085 Protein_1791 glutamate 5-kinase -
  QTV52_RS09290 - 1756520..1757284 (+) 765 WP_006248395.1 TSUP family transporter -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16841.15 Da        Isoelectric Point: 8.7964

>NTDB_id=848387 QTV52_RS09265 WP_006248401.1 1752386..1752865(-) (pilA) [Mannheimia haemolytica strain 171555]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=848387 QTV52_RS09265 WP_006248401.1 1752386..1752865(-) (pilA) [Mannheimia haemolytica strain 171555]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(48-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0S4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

56.429

88.05

0.497
  pilA Haemophilus influenzae Rd KW20

54.93

89.308

0.491