Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   QTV56_RS07810 Genome accession   NZ_CP128524
Coordinates   1532761..1533240 (+) Length   159 a.a.
NCBI ID   WP_006248401.1    Uniprot ID   A0A249A0S4
Organism   Mannheimia haemolytica strain 181789     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1527761..1538240
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QTV56_RS07785 - 1528342..1529106 (-) 765 WP_006248395.1 TSUP family transporter -
  QTV56_RS07790 proB 1529106..1530190 (-) 1085 Protein_1501 glutamate 5-kinase -
  QTV56_RS07795 folA 1530302..1530796 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  QTV56_RS07800 - 1530916..1531146 (+) 231 WP_006248398.1 YgjV family protein -
  QTV56_RS07805 radA 1531201..1532580 (-) 1380 WP_006248399.1 DNA repair protein RadA -
  QTV56_RS07810 pilA 1532761..1533240 (+) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  QTV56_RS07815 - 1533261..1533704 (+) 444 Protein_1506 pilus assembly protein PilB -
  QTV56_RS07820 pilC 1533710..1534906 (+) 1197 WP_006248403.1 type II secretion system F family protein Machinery gene
  QTV56_RS07825 - 1534909..1535565 (+) 657 WP_006248404.1 prepilin peptidase -
  QTV56_RS07830 coaE 1535601..1536236 (+) 636 WP_006248405.1 dephospho-CoA kinase -
  QTV56_RS07835 yacG 1536218..1536403 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  QTV56_RS07840 holA 1536404..1537429 (-) 1026 WP_006253426.1 DNA polymerase III subunit delta -
  QTV56_RS07845 lptE 1537472..1537960 (-) 489 WP_289332991.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16841.15 Da        Isoelectric Point: 8.7964

>NTDB_id=848364 QTV56_RS07810 WP_006248401.1 1532761..1533240(+) (pilA) [Mannheimia haemolytica strain 181789]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=848364 QTV56_RS07810 WP_006248401.1 1532761..1533240(+) (pilA) [Mannheimia haemolytica strain 181789]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(48-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0S4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

56.429

88.05

0.497
  pilA Haemophilus influenzae Rd KW20

54.93

89.308

0.491