Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   QTV63_RS04440 Genome accession   NZ_CP128522
Coordinates   864797..865276 (+) Length   159 a.a.
NCBI ID   WP_006248401.1    Uniprot ID   A0A249A0S4
Organism   Mannheimia haemolytica strain 182963     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 859797..870276
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QTV63_RS04415 - 860371..861135 (-) 765 WP_006248395.1 TSUP family transporter -
  QTV63_RS04420 proB 861135..862226 (-) 1092 WP_006251346.1 glutamate 5-kinase -
  QTV63_RS04425 folA 862338..862832 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  QTV63_RS04430 - 862952..863182 (+) 231 WP_006248398.1 YgjV family protein -
  QTV63_RS04435 radA 863237..864616 (-) 1380 WP_040080753.1 DNA repair protein RadA Machinery gene
  QTV63_RS04440 pilA 864797..865276 (+) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  QTV63_RS04445 pilB 865297..866697 (+) 1401 WP_040080755.1 GspE/PulE family protein Machinery gene
  QTV63_RS04450 pilC 866681..867877 (+) 1197 WP_230268904.1 type II secretion system F family protein Machinery gene
  QTV63_RS04455 - 867880..868536 (+) 657 WP_006248404.1 prepilin peptidase -
  QTV63_RS04460 coaE 868572..869207 (+) 636 WP_020831305.1 dephospho-CoA kinase -
  QTV63_RS04465 yacG 869189..869374 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16841.15 Da        Isoelectric Point: 8.7964

>NTDB_id=848307 QTV63_RS04440 WP_006248401.1 864797..865276(+) (pilA) [Mannheimia haemolytica strain 182963]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=848307 QTV63_RS04440 WP_006248401.1 864797..865276(+) (pilA) [Mannheimia haemolytica strain 182963]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(48-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0S4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

56.429

88.05

0.497
  pilA Haemophilus influenzae Rd KW20

54.93

89.308

0.491