Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   V5101_RS02065 Genome accession   NZ_CP144926
Coordinates   434021..434698 (-) Length   225 a.a.
NCBI ID   WP_000178157.1    Uniprot ID   -
Organism   Escherichia coli strain SCPM-O-B-7615     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 429021..439698
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V5101_RS02040 (V5101_02030) bfd 430710..430904 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  V5101_RS02045 (V5101_02035) - 430951..432564 (-) 1614 WP_000080195.1 IS66-like element ISEc23 family transposase -
  V5101_RS02050 (V5101_02040) tnpB 432595..432945 (-) 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
  V5101_RS02055 (V5101_02045) - 432942..433367 (-) 426 WP_000422741.1 transposase -
  V5101_RS02060 (V5101_02050) bfr 433516..433992 (+) 477 WP_000675504.1 bacterioferritin -
  V5101_RS02065 (V5101_02055) pilD 434021..434698 (-) 678 WP_000178157.1 prepilin peptidase GspO Machinery gene
  V5101_RS02070 (V5101_02060) gspM 434698..435159 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  V5101_RS02075 (V5101_02065) gspL 435156..436319 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  V5101_RS02080 (V5101_02070) gspK 436334..437317 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  V5101_RS02085 (V5101_02075) gspJ 437310..437897 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  V5101_RS02090 (V5101_02080) gspI 437890..438267 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  V5101_RS02095 (V5101_02085) gspH 438264..438773 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  V5101_RS02100 (V5101_02090) gspG 438781..439218 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24932.80 Da        Isoelectric Point: 7.9563

>NTDB_id=848221 V5101_RS02065 WP_000178157.1 434021..434698(-) (pilD) [Escherichia coli strain SCPM-O-B-7615]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGFVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=848221 V5101_RS02065 WP_000178157.1 434021..434698(-) (pilD) [Escherichia coli strain SCPM-O-B-7615]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTTGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378