Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   QTV68_RS04210 Genome accession   NZ_CP128516
Coordinates   811502..811981 (+) Length   159 a.a.
NCBI ID   WP_006248401.1    Uniprot ID   A0A249A0S4
Organism   Mannheimia haemolytica strain 192916     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 806502..816981
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QTV68_RS04185 - 807083..807847 (-) 765 WP_006248395.1 TSUP family transporter -
  QTV68_RS04190 proB 807847..808931 (-) 1085 Protein_803 glutamate 5-kinase -
  QTV68_RS04195 folA 809043..809537 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  QTV68_RS04200 - 809657..809887 (+) 231 WP_006248398.1 YgjV family protein -
  QTV68_RS04205 radA 809942..811321 (-) 1380 WP_006248399.1 DNA repair protein RadA -
  QTV68_RS04210 pilA 811502..811981 (+) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  QTV68_RS04215 - 812002..812445 (+) 444 Protein_808 pilus assembly protein PilB -
  QTV68_RS04220 pilC 812451..813647 (+) 1197 WP_006248403.1 type II secretion system F family protein Machinery gene
  QTV68_RS04225 - 813650..814306 (+) 657 WP_006248404.1 prepilin peptidase -
  QTV68_RS04230 coaE 814342..814977 (+) 636 WP_006248405.1 dephospho-CoA kinase -
  QTV68_RS04235 yacG 814959..815144 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  QTV68_RS04240 holA 815145..816170 (-) 1026 WP_006253426.1 DNA polymerase III subunit delta -
  QTV68_RS04245 lptE 816213..816701 (-) 489 WP_006248408.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16841.15 Da        Isoelectric Point: 8.7964

>NTDB_id=848214 QTV68_RS04210 WP_006248401.1 811502..811981(+) (pilA) [Mannheimia haemolytica strain 192916]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=848214 QTV68_RS04210 WP_006248401.1 811502..811981(+) (pilA) [Mannheimia haemolytica strain 192916]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(48-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0S4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

56.429

88.05

0.497
  pilA Haemophilus influenzae Rd KW20

54.93

89.308

0.491