Detailed information NTG0846047

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: QRD86_RS13655
• Genome accession: NZ_CP128116
• Coordinates: 2569484..2570101 (-)
• Length: 205 a.a.
• NCBI ID: WP_151175157.1
• Uniprot ID: -
• Organism: Bacillus halotolerans strain KF17
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2564484..2575101

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QRD86_RS13625 (QRD86_13620)	rpsT	2564787..2565053 (+)	267	WP_010335003.1	30S ribosomal protein S20	-
QRD86_RS13630 (QRD86_13625)	holA	2565068..2566111 (-)	1044	WP_151175155.1	DNA polymerase III subunit delta	-
QRD86_RS13635 (QRD86_13630)	-	2566151..2566300 (-)	150	WP_151175156.1	hypothetical protein	-
QRD86_RS13640 (QRD86_13635)	-	2566341..2566475 (+)	135	WP_010335005.1	YqzM family protein	-
QRD86_RS13645 (QRD86_13640)	comEC	2566514..2568844 (-)	2331	WP_286109244.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
QRD86_RS13650 (QRD86_13645)	-	2568848..2569417 (-)	570	WP_010335007.1	ComE operon protein 2	-
QRD86_RS13655 (QRD86_13650)	comEA	2569484..2570101 (-)	618	WP_151175157.1	helix-hairpin-helix domain-containing protein	Machinery gene
QRD86_RS13660 (QRD86_13655)	comER	2570185..2571006 (+)	822	WP_101864554.1	late competence protein ComER	-
QRD86_RS13665 (QRD86_13660)	-	2571071..2571814 (-)	744	WP_106020124.1	class I SAM-dependent methyltransferase	-
QRD86_RS13670 (QRD86_13665)	rsfS	2571811..2572167 (-)	357	WP_044154484.1	ribosome silencing factor	-
QRD86_RS13675 (QRD86_13670)	yqeK	2572185..2572745 (-)	561	WP_106020125.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
QRD86_RS13680 (QRD86_13675)	-	2572735..2573304 (-)	570	WP_010335014.1	nicotinate-nucleotide adenylyltransferase	-
QRD86_RS13685 (QRD86_13680)	yhbY	2573315..2573605 (-)	291	WP_106020126.1	ribosome assembly RNA-binding protein YhbY	-
QRD86_RS13690 (QRD86_13685)	aroE	2573605..2574441 (-)	837	WP_106022105.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 22045.89 Da
• Isoelectric Point: 4.8695

>NTDB_id=846047 QRD86_RS13655 WP_151175157.1 2569484..2570101(-) (comEA) [Bacillus halotolerans strain KF17]
MNWLNQHKTIIMSVGSAAVLIAVLCLFFFSGNNKEPVKQVVSSEKENEEVKQEATKDKAEENIVIDIKGAVKHPGVYEMH
TGDRVSQAIEKAGGIGEKADETQVNLAELLQDGTVVYIPSKGEEAAQQAAGGAVQSSKGKETLVNINTATLEDLQNISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=846047 QRD86_RS13655 WP_151175157.1 2569484..2570101(-) (comEA) [Bacillus halotolerans strain KF17]
TTGAATTGGTTGAATCAACATAAAACGATAATCATGTCAGTTGGCTCTGCTGCTGTTCTCATTGCAGTTCTTTGCCTCTT
TTTTTTCAGCGGAAATAACAAAGAGCCTGTAAAACAAGTTGTATCTAGTGAAAAGGAAAATGAGGAGGTCAAGCAGGAAG
CAACAAAAGACAAAGCGGAAGAAAATATTGTAATCGATATAAAAGGCGCTGTTAAGCATCCCGGCGTTTATGAAATGCAT
ACGGGAGACAGAGTATCGCAGGCTATTGAGAAAGCAGGCGGCATTGGTGAAAAAGCAGATGAAACGCAAGTGAATTTGGC
GGAACTTTTACAGGACGGTACAGTGGTGTATATACCGAGTAAGGGAGAAGAGGCGGCACAGCAAGCGGCCGGCGGCGCTG
TACAAAGCAGTAAGGGGAAGGAGACATTGGTCAATATCAATACAGCAACATTAGAGGATTTACAAAACATTTCGGGAGTA
GGACCTTCAAAAGCCGAAGCGATTATTGCGTATCGGGAAGAGAACGGGCGTTTCCAGACAATCGAAGATATCACAAAGGT
TTCGGGAATTGGCGAAAAATCTTTTGAAAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (64-119)

• Helix-hairpin-helix motif (HHH_3) (141-203)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	77.184	100	0.776
comEA	Staphylococcus aureus MW2	37.727	100	0.405
comEA	Staphylococcus aureus N315	37.387	100	0.405
comEA	Streptococcus thermophilus LMD-9	37.264	100	0.385
comEA	Latilactobacillus sakei subsp. sakei 23K	35.78	100	0.38