NTDB

Overview

Name	comFA	Type	Machinery gene
Locus tag	QRD88_RS16265	Genome accession	NZ_CP128114
Coordinates	3137079..3138476 (-)	Length	465 a.a.
NCBI ID	WP_286099159.1	Uniprot ID	-
Organism	Bacillus safensis strain G6S3
Function	ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3132079..3143476

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QRD88_RS16230 (QRD88_16225)	flgL	3132280..3133188 (-)	909	WP_024423617.1	flagellar hook-associated protein FlgL	-
QRD88_RS16235 (QRD88_16230)	flgK	3133198..3134718 (-)	1521	WP_286098769.1	flagellar hook-associated protein FlgK	-
QRD88_RS16240 (QRD88_16235)	-	3134734..3135216 (-)	483	WP_286098770.1	flagellar protein FlgN	-
QRD88_RS16245 (QRD88_16240)	flgM	3135235..3135498 (-)	264	WP_024423620.1	flagellar biosynthesis anti-sigma factor FlgM	-
QRD88_RS16250 (QRD88_16245)	-	3135578..3135997 (-)	420	WP_024423621.1	TIGR03826 family flagellar region protein	-
QRD88_RS16255 (QRD88_16250)	comFC	3136054..3136710 (-)	657	WP_241218534.1	ComF family protein	Machinery gene
QRD88_RS16260 (QRD88_16255)	-	3136737..3137030 (-)	294	WP_025093967.1	competence protein ComFB	-
QRD88_RS16265 (QRD88_16260)	comFA	3137079..3138476 (-)	1398	WP_286099159.1	DEAD/DEAH box helicase	Machinery gene
QRD88_RS16270 (QRD88_16265)	-	3138597..3139439 (-)	843	WP_024423625.1	DegV family protein	-
QRD88_RS16275 (QRD88_16270)	degU	3139655..3140344 (-)	690	WP_008348273.1	two-component system response regulator DegU	Regulator
QRD88_RS16280 (QRD88_16275)	degS	3140409..3141584 (-)	1176	WP_003213166.1	sensor histidine kinase	Regulator
QRD88_RS16285 (QRD88_16280)	-	3141805..3142473 (+)	669	WP_012011434.1	YigZ family protein	-

Sequence

Protein

Download Length: 465 a.a. Molecular weight: 53123.00 Da Isoelectric Point: 9.8362

                                >NTDB_id=845986 QRD88_RS16265 WP_286099159.1 3137079..3138476(-) (comFA) [Bacillus safensis strain G6S3]

MNMDKAMELMRHLHSRHLLTVETKCPQSNLDWLEEKGLVNRSPAIERKANGFTCCRCGVSHKRYFAHSPCEVCQKDCVYC

RSCIMMGKAAECGFLYEWTGPQMVETCRAELTWQGELSKGQKRASERMIEAIKNKFDLLVWAVCGAGKTEVLFHGIEYAL

NQGMSVCIATPRTDVVLELEPRLTKAFQGVTITVLYGGSSQRFQITPLVITTTHQLMRYKNAFDVLIVDEVDAFPYSMDE

RLQFAVLKAMRKNRGVRIYLSATPSKKMTREVSRGKLEAIKIPLRFHEQPLPVPTFHWIGHWKKKLKKNQLPPKVMNWMQ

RHIANKRRILLFVPSIATMKKVTKILREQSLNVEGVSADDPDRKQKVQHFRDYVYDVLVTTTILERGVTIPNVQVGVLGS

ESTIFTESALVQISGRVGRHPDYCTGDVFLFHFGLTRSMKQAKKHIAKMNDTAAKEFSEKQCGFN

Nucleotide

Download Length: 1398 bp

                                >NTDB_id=845986 QRD88_RS16265 WP_286099159.1 3137079..3138476(-) (comFA) [Bacillus safensis strain G6S3]

TTGAATATGGACAAAGCAATGGAACTAATGCGCCATCTGCATTCACGCCATCTGCTCACCGTTGAAACAAAGTGCCCACA

GTCCAATTTAGATTGGCTGGAGGAGAAGGGCTTAGTCAATCGGTCTCCAGCCATTGAGAGGAAAGCAAATGGTTTTACAT

GCTGCCGGTGCGGTGTATCACACAAGCGGTATTTCGCTCATTCTCCATGTGAGGTGTGTCAAAAGGATTGTGTGTACTGT

AGATCATGCATCATGATGGGAAAAGCAGCAGAATGTGGGTTTCTTTATGAATGGACAGGTCCACAAATGGTAGAAACATG

TCGAGCCGAATTAACATGGCAGGGAGAGCTGTCTAAAGGACAAAAAAGAGCGTCAGAAAGAATGATTGAAGCTATTAAAA

ACAAATTTGATCTACTTGTTTGGGCGGTTTGCGGAGCAGGGAAAACGGAAGTGCTTTTTCACGGAATCGAATATGCGTTA

AATCAAGGAATGAGTGTCTGTATTGCGACACCTAGAACAGATGTAGTGCTTGAACTCGAACCACGGCTTACAAAAGCATT

TCAAGGGGTGACAATTACCGTACTTTACGGAGGCAGTTCTCAAAGGTTTCAAATAACACCGCTTGTGATCACCACAACCC

ACCAGCTGATGAGATACAAAAATGCTTTTGATGTGCTCATTGTAGATGAAGTCGATGCCTTCCCTTATTCAATGGATGAG

CGTCTCCAATTTGCTGTTCTAAAGGCGATGAGAAAGAACAGAGGGGTCAGGATTTATTTAAGTGCGACACCCTCTAAAAA

AATGACGAGAGAGGTTTCTCGTGGAAAACTGGAAGCGATAAAAATTCCTTTGCGTTTTCACGAGCAACCATTACCCGTAC

CGACCTTTCATTGGATCGGTCATTGGAAAAAGAAATTAAAAAAGAACCAGCTGCCCCCTAAAGTGATGAATTGGATGCAG

AGACATATTGCAAATAAGAGAAGAATATTACTTTTTGTTCCTTCAATTGCTACCATGAAGAAGGTAACGAAGATTCTTCG

AGAACAATCCTTGAACGTGGAGGGGGTATCTGCCGATGATCCGGATAGAAAACAAAAGGTTCAGCACTTTAGAGATTACG

TATACGATGTACTAGTTACAACCACTATTCTAGAAAGAGGCGTAACGATTCCAAATGTTCAAGTGGGGGTACTAGGTTCG

GAATCTACTATTTTTACAGAGAGTGCACTTGTTCAGATTTCTGGGCGGGTAGGCAGACACCCGGATTATTGTACAGGAGA

TGTTTTCCTTTTTCATTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAGCATATCGCAAAAATGAATGATACGGCTG

CAAAAGAGTTTTCTGAAAAACAGTGTGGGTTCAACTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(324-420)

Type III restriction enzyme, res subunit (ResIII)

(116-265)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comFA	Bacillus subtilis subsp. subtilis str. 168	52.193	98.065	0.512
comFA/cflA	Streptococcus pneumoniae Rx1	38.095	94.839	0.361
comFA/cflA	Streptococcus pneumoniae D39	38.095	94.839	0.361
comFA/cflA	Streptococcus pneumoniae R6	38.095	94.839	0.361

Detailed information NTG0845986