Detailed information NTG0844113

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: QP482_RS16920
• Genome accession: NZ_CP127406
• Coordinates: 3697735..3699462 (-)
• Length: 575 a.a.
• NCBI ID: WP_205406020.1
• Uniprot ID: A0AAW4GGD5
• Organism: Stenotrophomonas sp. As-14
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	3700080..3711983	3697735..3699462	flank	618

Gene organization within MGE regions

Location: 3697735..3711983

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QP482_RS16920	pilB	3697735..3699462 (-)	1728	WP_205406020.1	type IV-A pilus assembly ATPase PilB	Machinery gene
QP482_RS16925	comP	3699576..3699986 (-)	411	WP_100553380.1	pilin	Machinery gene
QP482_RS16930	-	3700080..3700976 (-)	897	WP_205406021.1	serine hydrolase domain-containing protein	-
QP482_RS16935	-	3700997..3702109 (-)	1113	WP_205406022.1	isoprenylcysteine carboxylmethyltransferase family protein	-
QP482_RS16940	comP	3702280..3702723 (-)	444	WP_100553383.1	pilin	Machinery gene
QP482_RS16945	pilC	3703071..3704330 (+)	1260	WP_205406026.1	type II secretion system F family protein	Machinery gene
QP482_RS16950	-	3704339..3705202 (+)	864	WP_089236602.1	A24 family peptidase	-
QP482_RS16955	coaE	3705214..3705825 (+)	612	WP_180165743.1	dephospho-CoA kinase	-
QP482_RS16960	-	3705925..3706311 (-)	387	WP_100553386.1	hypothetical protein	-
QP482_RS16965	-	3706876..3708246 (-)	1371	WP_100460170.1	HAMP domain-containing sensor histidine kinase	-
QP482_RS16970	-	3708209..3708886 (-)	678	WP_025874286.1	response regulator transcription factor	-
QP482_RS16975	-	3708895..3709368 (-)	474	WP_180165745.1	hypothetical protein	-
QP482_RS16980	-	3709458..3709934 (-)	477	WP_205406023.1	hypothetical protein	-
QP482_RS16985	rimK	3710188..3711066 (-)	879	WP_100553389.1	30S ribosomal protein S6--L-glutamate ligase	-
QP482_RS16990	-	3711161..3711403 (-)	243	WP_180165749.1	hypothetical protein	-
QP482_RS16995	-	3711603..3711983 (-)	381	WP_180165751.1	DUF805 domain-containing protein	-

Sequence

Protein

• Length: 575 a.a.
• Molecular weight: 62276.30 Da
• Isoelectric Point: 6.1548

>NTDB_id=844113 QP482_RS16920 WP_205406020.1 3697735..3699462(-) (pilB) [Stenotrophomonas sp. As-14]
MNAATTANLVGITGLARRLVQDGAMDEVAARDAMAKATAARQPLPTWFAQNKVVGAAQLAAANSLEFGMPLFDVSTFDSS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTMGDALGGD
DDAMANLDIAGGDDEMASTDSGIDAKGDDTPVVKFINKVLIDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPTKL
NQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTRQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLFL
EAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDII
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCSNCKRPTDLPAHA
LLAEGFTQAQLDAGIQLHEAVGCDECTEGYKGRTGLYQVMPMTDEIATIVLAGGNALQIAEAAQAIGVNDLRQSALKKAA
AGVTSLAEINRVTKD

Nucleotide

• Length: 1728 bp

>NTDB_id=844113 QP482_RS16920 WP_205406020.1 3697735..3699462(-) (pilB) [Stenotrophomonas sp. As-14]
ATGAACGCAGCTACCACCGCCAACCTCGTCGGCATCACCGGTCTGGCCCGCCGCCTGGTACAGGACGGCGCCATGGATGA
AGTCGCTGCCCGCGACGCCATGGCCAAGGCCACCGCCGCGCGTCAGCCGCTGCCAACATGGTTTGCGCAGAACAAGGTCG
TCGGCGCGGCCCAGTTGGCCGCCGCCAACTCACTCGAGTTCGGCATGCCGCTGTTCGATGTGTCCACCTTCGACAGCAGC
CAGAACGCGATGAGCCTGGTCAGCGAGGAACTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAACGCGGTGGCAAGCT
GTTCGTCGGCACCAGCAACCCCACCCACTCGCTGGATGAGATCAAGTTCCACACCAATCTGGTGGTTGAGCCGATTCTGG
TGGATGAGGACCAGATCCGTCGCACGCTGGAGCAGTGGCATGCCAGCCACGACACCATGGGCGATGCGCTCGGCGGCGAC
GACGATGCCATGGCCAACCTCGACATCGCCGGTGGCGACGATGAGATGGCCAGCACCGACAGCGGCATCGACGCCAAGGG
CGACGACACCCCGGTGGTCAAGTTCATCAACAAGGTGCTGATCGACGCCATCCGCAAGGGCGCGTCGGACATTCACTTCG
AGCCCTACGAAGACGACTATCGCGTGCGCCTGCGCATCGACGGCCTGCTGAAGATGGTCGCGCGCGCGCCGACCAAGCTC
AACCAGCGCATCGCTGCGCGCCTGAAGGTGATGGCGCAGCTGGATATCGCGGAAAAGCGCGTGCCGCAGGATGGTCGCAT
CAAGCTCAACCTGTCCAAGACCCGGCAGATCGACTTCCGTGTCAGCACGTTGCCGACGCTGTTCGGTGAGAAGGTGGTGC
TGCGTATCCTCGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAGCAGAAGCTGTTCCTG
GAAGCGATCCACAAACCGTATGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTGTACACCGC
GCTGGGCATCCTCAACGACGAAACCCGCAATATTTCTACGGCCGAAGACCCGGTGGAAATCCGTCTGCCCGGCGTCAACC
AGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCAGCCCTGCGCTCGTTCCTGCGACAGGATCCGGACATCATC
ATGGTCGGCGAAATCCGCGACCTGGAAACGGCGGAAATTGCGATCAAGGCCGCACAGACCGGTCACATGGTGCTGTCCAC
GCTGCATACCAACGACGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCACCAGCTCGG
TGACGCTGGTGATCGCCCAGCGCTTGGCGCGCCGCCTGTGTTCGAACTGCAAGCGGCCGACAGACCTGCCCGCGCATGCG
CTGCTGGCAGAAGGCTTCACCCAGGCGCAGCTGGATGCGGGCATCCAGCTGCACGAAGCGGTCGGCTGCGATGAATGCAC
CGAGGGCTACAAGGGCCGTACCGGTCTCTACCAGGTGATGCCGATGACCGACGAGATCGCGACCATCGTGCTGGCGGGCG
GCAACGCGCTGCAGATTGCCGAGGCTGCGCAGGCGATCGGTGTGAACGACCTGCGGCAGTCGGCGTTGAAGAAGGCGGCT
GCAGGTGTGACCAGCCTGGCCGAGATTAACCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (198-465)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (49-150)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baylyi ADP1	55.882	100	0.562
pilB	Acinetobacter baumannii D1279779	56.415	98.957	0.558
pilB	Legionella pneumophila strain ERS1305867	51.408	98.783	0.508
pilF	Neisseria gonorrhoeae MS11	49.12	98.783	0.485
pilB	Vibrio cholerae strain A1552	49.033	98.957	0.485
pilB	Vibrio parahaemolyticus RIMD 2210633	46.06	99.304	0.457
pilB	Vibrio campbellii strain DS40M4	44.93	99.478	0.447
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.043	96	0.365