Detailed information NTG0844059

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: QP377_RS17145
• Genome accession: NZ_CP127403
• Coordinates: 3745973..3747700 (-)
• Length: 575 a.a.
• NCBI ID: WP_205406020.1
• Uniprot ID: A0AAW4GGD5
• Organism: Stenotrophomonas sp. As-5
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	3748318..3760221	3745973..3747700	flank	618

Gene organization within MGE regions

Location: 3745973..3760221

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QP377_RS17145	pilB	3745973..3747700 (-)	1728	WP_205406020.1	type IV-A pilus assembly ATPase PilB	Machinery gene
QP377_RS17150	comP	3747814..3748224 (-)	411	WP_100553380.1	pilin	Machinery gene
QP377_RS17155	-	3748318..3749214 (-)	897	WP_205406021.1	serine hydrolase domain-containing protein	-
QP377_RS17160	-	3749235..3750347 (-)	1113	WP_205406022.1	isoprenylcysteine carboxylmethyltransferase family protein	-
QP377_RS17165	comP	3750518..3750961 (-)	444	WP_100553383.1	pilin	Machinery gene
QP377_RS17170	pilC	3751309..3752568 (+)	1260	WP_205406026.1	type II secretion system F family protein	Machinery gene
QP377_RS17175	-	3752577..3753440 (+)	864	WP_089236602.1	A24 family peptidase	-
QP377_RS17180	coaE	3753452..3754063 (+)	612	WP_180165743.1	dephospho-CoA kinase	-
QP377_RS17185	-	3754163..3754549 (-)	387	WP_100553386.1	hypothetical protein	-
QP377_RS17190	-	3755114..3756484 (-)	1371	WP_100460170.1	HAMP domain-containing sensor histidine kinase	-
QP377_RS17195	-	3756447..3757124 (-)	678	WP_025874286.1	response regulator transcription factor	-
QP377_RS17200	-	3757133..3757606 (-)	474	WP_180165745.1	hypothetical protein	-
QP377_RS17205	-	3757696..3758172 (-)	477	WP_205406023.1	hypothetical protein	-
QP377_RS17210	rimK	3758426..3759304 (-)	879	WP_100553389.1	30S ribosomal protein S6--L-glutamate ligase	-
QP377_RS17215	-	3759399..3759641 (-)	243	WP_180165749.1	hypothetical protein	-
QP377_RS17220	-	3759841..3760221 (-)	381	WP_180165751.1	DUF805 domain-containing protein	-

Sequence

Protein

• Length: 575 a.a.
• Molecular weight: 62276.30 Da
• Isoelectric Point: 6.1548

>NTDB_id=844059 QP377_RS17145 WP_205406020.1 3745973..3747700(-) (pilB) [Stenotrophomonas sp. As-5]
MNAATTANLVGITGLARRLVQDGAMDEVAARDAMAKATAARQPLPTWFAQNKVVGAAQLAAANSLEFGMPLFDVSTFDSS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTMGDALGGD
DDAMANLDIAGGDDEMASTDSGIDAKGDDTPVVKFINKVLIDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPTKL
NQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTRQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLFL
EAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDII
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCSNCKRPTDLPAHA
LLAEGFTQAQLDAGIQLHEAVGCDECTEGYKGRTGLYQVMPMTDEIATIVLAGGNALQIAEAAQAIGVNDLRQSALKKAA
AGVTSLAEINRVTKD

Nucleotide

• Length: 1728 bp

>NTDB_id=844059 QP377_RS17145 WP_205406020.1 3745973..3747700(-) (pilB) [Stenotrophomonas sp. As-5]
ATGAACGCAGCTACCACCGCCAACCTCGTCGGCATCACCGGTCTGGCCCGCCGCCTGGTACAGGACGGCGCCATGGATGA
AGTCGCTGCCCGCGACGCCATGGCCAAGGCCACCGCCGCGCGTCAGCCGCTGCCAACATGGTTTGCGCAGAACAAGGTCG
TCGGCGCGGCCCAGTTGGCCGCCGCCAACTCACTCGAGTTCGGCATGCCGCTGTTCGATGTGTCCACCTTCGACAGCAGC
CAGAACGCGATGAGCCTGGTCAGCGAGGAACTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAACGCGGTGGCAAGCT
GTTCGTCGGCACCAGCAACCCCACCCACTCGCTGGATGAGATCAAGTTCCACACCAATCTGGTGGTTGAGCCGATTCTGG
TGGATGAGGACCAGATCCGTCGCACGCTGGAGCAGTGGCATGCCAGCCACGACACCATGGGCGATGCGCTCGGCGGCGAC
GACGATGCCATGGCCAACCTCGACATCGCCGGTGGCGACGATGAGATGGCCAGCACCGACAGCGGCATCGACGCCAAGGG
CGACGACACCCCGGTGGTCAAGTTCATCAACAAGGTGCTGATCGACGCCATCCGCAAGGGCGCGTCGGACATTCACTTCG
AGCCCTACGAAGACGACTATCGCGTGCGCCTGCGCATCGACGGCCTGCTGAAGATGGTCGCGCGCGCGCCGACCAAGCTC
AACCAGCGCATCGCTGCGCGCCTGAAGGTGATGGCGCAGCTGGATATCGCGGAAAAGCGCGTGCCGCAGGATGGTCGCAT
CAAGCTCAACCTGTCCAAGACCCGGCAGATCGACTTCCGTGTCAGCACGTTGCCGACGCTGTTCGGTGAGAAGGTGGTGC
TGCGTATCCTCGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAGCAGAAGCTGTTCCTG
GAAGCGATCCACAAACCGTATGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTGTACACCGC
GCTGGGCATCCTCAACGACGAAACCCGCAATATTTCTACGGCCGAAGACCCGGTGGAAATCCGTCTGCCCGGCGTCAACC
AGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCAGCCCTGCGCTCGTTCCTGCGACAGGATCCGGACATCATC
ATGGTCGGCGAAATCCGCGACCTGGAAACGGCGGAAATTGCGATCAAGGCCGCACAGACCGGTCACATGGTGCTGTCCAC
GCTGCATACCAACGACGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCACCAGCTCGG
TGACGCTGGTGATCGCCCAGCGCTTGGCGCGCCGCCTGTGTTCGAACTGCAAGCGGCCGACAGACCTGCCCGCGCATGCG
CTGCTGGCAGAAGGCTTCACCCAGGCGCAGCTGGATGCGGGCATCCAGCTGCACGAAGCGGTCGGCTGCGATGAATGCAC
CGAGGGCTACAAGGGCCGTACCGGTCTCTACCAGGTGATGCCGATGACCGACGAGATCGCGACCATCGTGCTGGCGGGCG
GCAACGCGCTGCAGATTGCCGAGGCTGCGCAGGCGATCGGTGTGAACGACCTGCGGCAGTCGGCGTTGAAGAAGGCGGCT
GCAGGTGTGACCAGCCTGGCCGAGATTAACCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (198-465)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (49-150)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baylyi ADP1	55.882	100	0.562
pilB	Acinetobacter baumannii D1279779	56.415	98.957	0.558
pilB	Legionella pneumophila strain ERS1305867	51.408	98.783	0.508
pilF	Neisseria gonorrhoeae MS11	49.12	98.783	0.485
pilB	Vibrio cholerae strain A1552	49.033	98.957	0.485
pilB	Vibrio parahaemolyticus RIMD 2210633	46.06	99.304	0.457
pilB	Vibrio campbellii strain DS40M4	44.93	99.478	0.447
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.043	96	0.365