Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   QAA96_RS29895 Genome accession   NZ_CP143646
Coordinates   6394273..6394896 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain CPO174     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6389273..6399896
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QAA96_RS29875 (QAA96_029875) aroB 6389868..6390974 (-) 1107 WP_003162482.1 3-dehydroquinate synthase -
  QAA96_RS29880 (QAA96_029880) aroK 6391024..6391542 (-) 519 WP_003095833.1 shikimate kinase AroK -
  QAA96_RS29885 (QAA96_029885) pilQ 6391554..6393698 (-) 2145 WP_285239537.1 type 4a pilus secretin PilQ Machinery gene
  QAA96_RS29890 (QAA96_029890) comL/pilP 6393752..6394276 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  QAA96_RS29895 (QAA96_029895) pilO 6394273..6394896 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  QAA96_RS29900 (QAA96_029900) pilN 6394893..6395489 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  QAA96_RS29905 (QAA96_029905) pilM 6395489..6396553 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  QAA96_RS29910 (QAA96_029910) - 6396738..6399206 (+) 2469 WP_025297613.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=843932 QAA96_RS29895 WP_003103268.1 6394273..6394896(-) (pilO) [Pseudomonas aeruginosa strain CPO174]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=843932 QAA96_RS29895 WP_003103268.1 6394273..6394896(-) (pilO) [Pseudomonas aeruginosa strain CPO174]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTTGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTATATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401