Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   N024_RS01630 Genome accession   NZ_CP127246
Coordinates   344625..345689 (+) Length   354 a.a.
NCBI ID   WP_003365992.1    Uniprot ID   A0A193SY06
Organism   Pseudomonas syringae CC457     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 339625..350689
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N024_RS01620 (N024_01620) - 339861..341129 (+) 1269 WP_003365988.1 malic enzyme-like NAD(P)-binding protein -
  N024_RS01625 (N024_01625) - 341974..344418 (-) 2445 WP_024662381.1 penicillin-binding protein 1A -
  N024_RS01630 (N024_01630) pilM 344625..345689 (+) 1065 WP_003365992.1 pilus assembly protein PilM Machinery gene
  N024_RS01635 (N024_01635) - 345689..346264 (+) 576 WP_003365994.1 PilN domain-containing protein -
  N024_RS01640 (N024_01640) pilO 346261..346884 (+) 624 WP_003432147.1 type 4a pilus biogenesis protein PilO -
  N024_RS01645 (N024_01645) pilP 346881..347408 (+) 528 WP_003350151.1 type 4a pilus biogenesis lipoprotein PilP -
  N024_RS01650 (N024_01650) pilQ 347456..349561 (+) 2106 WP_024662382.1 type IV pilus secretin PilQ Machinery gene
  N024_RS01655 (N024_01655) aroK 349566..350084 (+) 519 WP_003365999.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38104.65 Da        Isoelectric Point: 4.7049

>NTDB_id=843054 N024_RS01630 WP_003365992.1 344625..345689(+) (pilM) [Pseudomonas syringae CC457]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKEALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSAKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=843054 N024_RS01630 WP_003365992.1 344625..345689(+) (pilM) [Pseudomonas syringae CC457]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACCCGTTACAAGGTCGAGTCTTACGCAGTAGAGCCCTTGCCGGCCAACGCTGTCGTCGAAAAGAACA
TTGCCGAACTCGAAGGGGTCGGGCAGGCGTTGTCACGCGTACTCGTCAAGGCCAAGACCAGCGTCAAGATCGTGGCGGTT
GCCGTAGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGACGCCGGTCTTTCCGACGATGACATGGAAAACCAGCT
CAAGCTTGAGGCTGACCAGTACATCCCCTATCCACTGGAAGAAGTCGCCATCGATTTCGAAGTGCAGGGCTACTCGGTGC
GCAACCCCGAGCGCGTCGAGGTGTTGCTGGCTGCCTGCCGCAAGGAGAACGTCGAAGTACGCGAGGCCGCGCTGGCACTG
GCCGGGCTGACCGCGCGCGTCGTCGATGTCGAGGCCTACGCCCTTGAGCGCTCGTTCGGGCTGCTGGCTGCGCAACTGGG
CAACGGCCATGACGAACTGACGGTGGCCGTGGTCGATATCGGCGCCACCATGACCACCCTGAGCGTGCTGCATCACGGCC
GGATCATCTATACCCGCGAGCAGCTGTTTGGCGGGCGCCAGCTGACTGACGAAATCCAGCGCCGCTATGGGCTCTCCATG
GAGGAAGCCGGGCTTGCCAAGAAGCAGGGCGGCTTGCCCGACGACTACGTCAGCGAAGTGCTCGACCCCTTCAAGGAAGC
GCTGGTGCAGCAGGTTTCCCGCTCCCTGCAGTTCTTCTTCGCGGCCGGTCAATACAACTCTGTGGATCACATCATGCTGG
CAGGCGGCACGGCTTCGATTTCAGGGCTGGAGCATCTGATCCAGCGACGCATCGGCACTCCGACCATGGTCGCCAACCCG
TTTGCCGACATGGCCCTGAGCGCCAAGGTCAATGCCGGGGCCCTGGCCAGTGACGCGCCAGCCCTGATGATCGCCTGTGG
CCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A193SY06

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

53.955

100

0.54
  pilM Legionella pneumophila strain ERS1305867

46.479

100

0.466