Detailed information NTG0084244

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: COW1_RS10535
• Genome accession: NZ_AP024239
• Coordinates: 2250193..2251371 (+)
• Length: 392 a.a.
• NCBI ID: WP_096364588.1
• Uniprot ID: A0A1Z4VN36
• Organism: Thiohalobacter sp. COW1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2245193..2256371

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
COW1_RS10515 (TspCOW1_20810)	-	2245289..2245513 (-)	225	WP_096364592.1	zinc-finger domain-containing protein	-
COW1_RS10520 (TspCOW1_20820)	-	2245898..2246818 (-)	921	WP_201344140.1	branched-chain amino acid transaminase	-
COW1_RS10525 (TspCOW1_20830)	glnE	2246920..2249826 (-)	2907	WP_242466420.1	bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase	-
COW1_RS10530 (TspCOW1_20840)	-	2249922..2250131 (-)	210	WP_201344141.1	hypothetical protein	-
COW1_RS10535 (TspCOW1_20850)	pilU	2250193..2251371 (+)	1179	WP_096364588.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
COW1_RS10540 (TspCOW1_20860)	-	2251866..2252429 (+)	564	WP_096364586.1	RNA polymerase sigma factor	-
COW1_RS10545 (TspCOW1_20870)	-	2252429..2253199 (+)	771	WP_201344142.1	hypothetical protein	-
COW1_RS10550 (TspCOW1_20880)	groL	2253566..2255218 (-)	1653	WP_096364582.1	chaperonin GroEL	-
COW1_RS10555 (TspCOW1_20890)	groES	2255264..2255554 (-)	291	WP_096364580.1	co-chaperone GroES	-

Sequence

Protein

• Length: 392 a.a.
• Molecular weight: 43850.01 Da
• Isoelectric Point: 5.1153

>NTDB_id=84244 COW1_RS10535 WP_096364588.1 2250193..2251371(+) (pilU) [Thiohalobacter sp. COW1]
MEFKDYLKIMVMKDASDLYLSTGAPPCAKFHGQLKVVEKATMTPERIREIAYSLMSETQQKDFEKKPEMNLAISEHNVGR
FRVNIFKQRNSIGMVVRNIKTDIPAWQDLGLPPILTDVLMSKRGLVLFVGATGSGKSTSLASLIDYRNTNATGHIITIED
PIEFIHSHKKSIVNQREVGLDTDSYEDALANTLRQAPDVILIGEIRHRETMEHAISFAETGHLCLSTLHANNANQALDRI
INFFPEEKRDQLLLDLSLNLRAFISQRLVQTVDGQRTAAIEILLGTPLVKELVRRGDVHEIKEVMEKSENLGMQTFDMAL
YNLYMEGRISLEEALRNADSPNNLRLRINLTAEDAEQALAEKSESFSLEEDDTPSSNIATAGGGLGLKLADE

Nucleotide

• Length: 1179 bp

>NTDB_id=84244 COW1_RS10535 WP_096364588.1 2250193..2251371(+) (pilU) [Thiohalobacter sp. COW1]
ATGGAATTCAAGGATTATCTCAAGATCATGGTCATGAAGGACGCCTCCGACCTCTACCTGAGCACGGGGGCGCCGCCCTG
CGCCAAGTTCCATGGCCAACTCAAGGTGGTGGAGAAGGCCACCATGACGCCGGAGCGCATCCGCGAGATCGCCTACTCGC
TGATGAGCGAAACCCAGCAGAAGGACTTCGAGAAGAAGCCGGAAATGAACCTGGCCATCTCCGAGCACAATGTCGGCCGC
TTCCGCGTCAACATCTTCAAGCAGCGCAACAGCATCGGCATGGTGGTGCGCAACATCAAGACCGATATCCCGGCGTGGCA
GGACCTGGGGCTGCCGCCCATCCTCACCGACGTGTTGATGAGCAAGCGCGGCCTGGTGCTGTTCGTGGGCGCGACCGGCT
CGGGCAAGTCCACCTCGCTGGCCTCGCTGATCGACTATCGCAACACCAACGCCACCGGCCACATCATCACCATCGAGGAT
CCGATCGAGTTCATCCACTCGCACAAGAAGTCCATCGTCAACCAGCGCGAGGTCGGGCTGGACACCGATTCCTATGAAGA
CGCCCTGGCCAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCCATCGCGAGACCATGGAGCATG
CCATCTCCTTCGCCGAGACCGGCCACCTGTGCCTGTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAGGAGAAGCGCGACCAGTTGCTGCTGGACCTGTCGCTGAACCTGCGCGCCTTCATCTCCCAGCG
CCTGGTGCAGACCGTCGACGGCCAGCGCACCGCGGCGATCGAGATCCTGCTGGGCACCCCGCTGGTCAAGGAACTGGTGC
GCCGCGGCGATGTGCATGAGATCAAGGAGGTCATGGAGAAATCCGAGAACCTCGGCATGCAGACCTTCGACATGGCGCTG
TACAACCTCTACATGGAAGGCCGGATCAGCCTGGAAGAGGCGCTGCGCAATGCCGACTCCCCCAACAACCTGCGCCTGCG
CATCAATCTCACGGCCGAGGACGCCGAGCAGGCCCTGGCCGAGAAGAGCGAATCCTTCAGCCTGGAGGAGGACGATACCC
CGAGCAGCAACATCGCCACCGCCGGCGGCGGGCTGGGGTTGAAGCTGGCGGACGAATGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (14-270)

3D structure

• AlphaFold DB: A0A1Z4VN36

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.355	96.173	0.561
pilU	Acinetobacter baylyi ADP1	54.648	90.561	0.495
pilU	Vibrio cholerae strain A1552	52.45	88.52	0.464
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.239	100	0.423