Detailed information NTG0841935

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QQX47_RS02050
• Genome accession: NZ_CP127122
• Coordinates: 440786..441934 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain Ned_5_BIM
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 435786..446934

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QQX47_RS02025 (QQX47_02025)	-	436937..437233 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
QQX47_RS02030 (QQX47_02030)	-	437230..437823 (-)	594	WP_003084544.1	YggT family protein	-
QQX47_RS02035 (QQX47_02035)	proC	437834..438655 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
QQX47_RS02040 (QQX47_02040)	-	438667..439359 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
QQX47_RS02045 (QQX47_02045)	pilT	439574..440608 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
QQX47_RS02050 (QQX47_02050)	pilU	440786..441934 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
QQX47_RS02055 (QQX47_02055)	czcD	441942..442841 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
QQX47_RS02060 (QQX47_02060)	-	442995..443399 (+)	405	WP_003084560.1	NINE protein	-
QQX47_RS02065 (QQX47_02065)	-	443642..445015 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
QQX47_RS02070 (QQX47_02070)	-	445012..446196 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=841935 QQX47_RS02050 WP_003100959.1 440786..441934(+) (pilU) [Pseudomonas aeruginosa strain Ned_5_BIM]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=841935 QQX47_RS02050 WP_003100959.1 440786..441934(+) (pilU) [Pseudomonas aeruginosa strain Ned_5_BIM]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382