Detailed information NTG0841466

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: QO021_RS25300
• Genome accession: NZ_CP127045
• Coordinates: 5488074..5489138 (-)
• Length: 354 a.a.
• NCBI ID: WP_002551652.1
• Uniprot ID: A0A7Z6Y2Y4
• Organism: Pseudomonas amygdali pv. lachrymans strain YM7902
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5483074..5494138

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QO021_RS25275 (QO021_25270)	aroK	5483655..5484173 (-)	519	WP_002551657.1	shikimate kinase AroK	-
QO021_RS25280 (QO021_25275)	pilQ	5484178..5486283 (-)	2106	WP_005743117.1	type IV pilus secretin PilQ	Machinery gene
QO021_RS25285 (QO021_25280)	pilP	5486331..5486858 (-)	528	WP_002551655.1	type 4a pilus biogenesis lipoprotein PilP	-
QO021_RS25290 (QO021_25285)	pilO	5486855..5487478 (-)	624	WP_002551654.1	type 4a pilus biogenesis protein PilO	-
QO021_RS25295 (QO021_25290)	-	5487475..5488074 (-)	600	WP_004654254.1	PilN domain-containing protein	-
QO021_RS25300 (QO021_25295)	pilM	5488074..5489138 (-)	1065	WP_002551652.1	pilus assembly protein PilM	Machinery gene
QO021_RS25305 (QO021_25300)	-	5489345..5491789 (+)	2445	WP_005743118.1	penicillin-binding protein 1A	-
QO021_RS25310 (QO021_25305)	-	5492533..5493801 (-)	1269	WP_002551650.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38078.57 Da
• Isoelectric Point: 4.7008

>NTDB_id=841466 QO021_RS25300 WP_002551652.1 5488074..5489138(-) (pilM) [Pseudomonas amygdali pv. lachrymans strain YM7902]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLAKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=841466 QO021_RS25300 WP_002551652.1 5488074..5489138(-) (pilM) [Pseudomonas amygdali pv. lachrymans strain YM7902]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTACGCAGTCGAGCCGTTGCCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAGCTCGAAGGGGTTGGCCAGGCGCTGTCTCGCGTGCTCGCCAAGGCCAAGACCAGCGTCAAGATCGTGGCGGTC
GCCGTAGCCGGTTCCGCCGTGATCACCAAGACGATTGAAATGGATGCCGGTCTTTCCGACGACGACATGGAAAACCAGCT
CAAGCTTGAGGCTGACCAGTACATCCCTTATCCGCTGGAAGAAGTCGCCATCGATTTCGAAGTGCAGGGCTACTCGGTGC
GCAATCCCGAACGCGTCGAAGTATTGCTGGCTGCCTGCCGCAAAGAGAACGTCGAAGTGCGTGAGGCGGCGCTGGCTCTG
GCCGGGCTGACCGCGCGCGTGGTGGACGTCGAGGCCTATGCCCTGGAGCGCTCGTTCGGCCTGCTGGCTGCGCAACTGGG
TAACGGTCATGACGAGCTTACCGTGGCGGTGGTCGATATCGGTGCCACCATGACCACCCTCAGCGTGCTGCATCATGGCC
GAATCATCTATACCCGCGAGCAACTGTTTGGTGGCCGCCAGCTGACTGACGAAATCCAGCGCCGCTATGGCCTTTCCATG
GAGGAGGCCGGGCTTGCCAAGAAGCAGGGCGGATTGCCCGACGACTATGTCAGCGAGGTGCTGGACCCCTTCAAGGATGC
GTTGGTGCAGCAGGTCTCCCGTTCTTTGCAGTTCTTCTTTGCAGCCGGTCAATACAACTCCGTGGATCACATCATGCTGG
CAGGCGGCACGGCTTCGATTTCAGGGCTGGAGCATTTGATTCAACGACGCATTGGCACCCCGACGATGGTCGCTAACCCG
TTTGCCGACATGGCCCTGAGCTCCAAGGTCAATGCCGGGGCGCTGGCCAGTGACGCTCCCGCGTTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A7Z6Y2Y4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.215	100	0.562
comM	Acinetobacter nosocomialis M2	56.215	100	0.562
comM	Acinetobacter baylyi ADP1	53.672	100	0.537
pilM	Legionella pneumophila strain ERS1305867	46.761	100	0.469