Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   LLC_RS09350 Genome accession   NZ_AP024222
Coordinates   1848622..1849047 (+) Length   141 a.a.
NCBI ID   WP_014735174.1    Uniprot ID   -
Organism   Lactococcus cremoris strain EPSC     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1849253..1861784 1848622..1849047 flank 206


Gene organization within MGE regions


Location: 1848622..1861784
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLC_RS09350 (LLC_19270) comGF 1848622..1849047 (+) 426 WP_014735174.1 competence type IV pilus minor pilin ComGF Machinery gene
  LLC_RS09355 (LLC_19290) comGG 1849143..1849370 (+) 228 WP_228764408.1 competence protein ComGG Machinery gene
  LLC_RS09360 (LLC_19300) - 1849450..1849887 (+) 438 WP_011677180.1 zinc-dependent MarR family transcriptional regulator -
  LLC_RS09365 (LLC_19310) - 1849884..1850726 (+) 843 WP_011677179.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  LLC_RS09370 (LLC_19320) - 1850905..1851642 (+) 738 WP_011677178.1 metal ABC transporter ATP-binding protein -
  LLC_RS09375 (LLC_19330) - 1851635..1852444 (+) 810 WP_011677177.1 metal ABC transporter permease -
  LLC_RS09380 - 1852517..1853910 (+) 1394 WP_126354656.1 IS3 family transposase -
  LLC_RS09385 (LLC_19360) - 1853974..1854846 (-) 873 WP_014573331.1 RluA family pseudouridine synthase -
  LLC_RS09390 - 1855040..1855417 (+) 378 Protein_1854 pyridoxamine 5'-phosphate oxidase family protein -
  LLC_RS09395 (LLC_19380) - 1855532..1855963 (+) 432 WP_011677172.1 DUF3290 domain-containing protein -
  LLC_RS09400 (LLC_19390) - 1855981..1856616 (+) 636 WP_011677171.1 DUF421 domain-containing protein -
  LLC_RS09405 (LLC_19400) - 1857002..1859233 (+) 2232 WP_061777877.1 PBP1A family penicillin-binding protein -
  LLC_RS09410 (LLC_19410) - 1859335..1861152 (+) 1818 WP_126354658.1 acyltransferase family protein -
  LLC_RS09415 (LLC_19420) rpmG 1861193..1861342 (+) 150 WP_011677168.1 50S ribosomal protein L33 -
  LLC_RS09420 (LLC_19430) secE 1861454..1861648 (+) 195 WP_011677167.1 preprotein translocase subunit SecE -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 16210.78 Da        Isoelectric Point: 8.4549

>NTDB_id=84144 LLC_RS09350 WP_014735174.1 1848622..1849047(+) (comGF) [Lactococcus cremoris strain EPSC]
MKLKIRAFTLLECLVALLAISGSVLVISGLTRMIEEQMKISQNDSRKDWQIFCEQMRSELSGAKLDNVNQNFLYVTKDKK
LRFGLVGDDFRKSDDKGQGYQPMLYDLKGAKIQAEENLIKITIDFDNGGERVFIYRFTDTK

Nucleotide


Download         Length: 426 bp        

>NTDB_id=84144 LLC_RS09350 WP_014735174.1 1848622..1849047(+) (comGF) [Lactococcus cremoris strain EPSC]
TTGAAGCTCAAAATTAGGGCATTTACTTTACTTGAATGTCTCGTAGCACTTTTGGCAATTTCTGGTTCAGTGCTAGTCAT
CTCAGGGCTGACTAGAATGATTGAAGAACAGATGAAAATAAGCCAGAATGATAGTAGGAAAGATTGGCAAATATTTTGTG
AACAAATGCGTTCAGAACTCTCCGGAGCAAAATTAGACAATGTGAATCAAAATTTTTTGTATGTGACCAAAGATAAGAAG
TTACGGTTCGGATTAGTAGGGGATGATTTTCGTAAAAGTGATGATAAAGGGCAAGGATACCAACCGATGCTTTATGATTT
AAAAGGAGCAAAAATTCAGGCAGAAGAAAATTTGATAAAAATAACAATTGATTTTGATAATGGAGGTGAGCGAGTATTTA
TTTATCGATTTACTGATACAAAGTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(38-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Lactococcus lactis subsp. cremoris KW2

100

100

1
  comYF Streptococcus mutans UA140

43.972

100

0.44
  comYF Streptococcus mutans UA159

43.262

100

0.433
  comGF/cglF Streptococcus mitis NCTC 12261

39.286

99.291

0.39
  comGF/cglF Streptococcus mitis SK321

39.286

99.291

0.39
  comGF/cglF Streptococcus pneumoniae Rx1

37.857

99.291

0.376
  comGF/cglF Streptococcus pneumoniae D39

37.857

99.291

0.376
  comGF/cglF Streptococcus pneumoniae R6

37.857

99.291

0.376
  comGF/cglF Streptococcus pneumoniae TIGR4

37.857

99.291

0.376

Multiple sequence alignment