Detailed information NTG0841140

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: QQF68_RS24685
• Genome accession: NZ_CP126992
• Coordinates: 5525994..5527058 (-)
• Length: 354 a.a.
• NCBI ID: WP_005765505.1
• Uniprot ID: A0A3M3ZC03
• Organism: Pseudomonas syringae pv. antirrhini str. 126
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5520994..5532058

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QQF68_RS24660 (QQF68_24660)	aroK	5521548..5522066 (-)	519	WP_007245596.1	shikimate kinase AroK	-
QQF68_RS24665 (QQF68_24665)	pilQ	5522071..5524176 (-)	2106	WP_005765499.1	type IV pilus secretin PilQ	Machinery gene
QQF68_RS24670 (QQF68_24670)	pilP	5524224..5524751 (-)	528	WP_003378849.1	type 4a pilus biogenesis lipoprotein PilP	-
QQF68_RS24675 (QQF68_24675)	pilO	5524748..5525371 (-)	624	WP_005765501.1	type 4a pilus biogenesis protein PilO	-
QQF68_RS24680 (QQF68_24680)	-	5525368..5525994 (-)	627	WP_005765503.1	PilN domain-containing protein	-
QQF68_RS24685 (QQF68_24685)	pilM	5525994..5527058 (-)	1065	WP_005765505.1	pilus assembly protein PilM	Machinery gene
QQF68_RS24690 (QQF68_24690)	-	5527265..5529709 (+)	2445	WP_005765507.1	penicillin-binding protein 1A	-
QQF68_RS24695 (QQF68_24695)	-	5529911..5531179 (-)	1269	WP_003378855.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38117.65 Da
• Isoelectric Point: 4.7008

>NTDB_id=841140 QQF68_RS24685 WP_005765505.1 5525994..5527058(-) (pilM) [Pseudomonas syringae pv. antirrhini str. 126]
MFELFGKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLIKAKTNVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=841140 QQF68_RS24685 WP_005765505.1 5525994..5527058(-) (pilM) [Pseudomonas syringae pv. antirrhini str. 126]
GTGTTCGAACTCTTTGGTAAGAAGGCCAATACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTACGCAGTCGAGCCGTTACCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAACTCGAAGGGGTTGGTCAGGCGCTGTCTCGCGTACTGATCAAGGCCAAGACCAACGTCAAGATTGTGGCGGTT
GCCGTGGCGGGTTCCGCGGTGATCACCAAAACCATCGAGATGGATGCCGGTCTCTCCGACGACGACATGGAAAATCAGCT
CAAGCTTGAAGCTGATCAGTACATCCCTTACCCGCTCGAAGAAGTTGCCATCGATTTTGAAGTGCAGGGCTACTCGGTCC
GCAACCCGGAACGGGTCGAAGTCCTGCTGGCGGCCTGCCGCAAGGAAAACGTCGAAGTGCGCGAGGCCGCGCTGGCCCTG
GCCGGGCTGACGGCGCGTGTCGTGGACGTCGAGGCCTACGCGCTGGAGCGTTCATTCGGTCTGCTGGCTGCGCAACTGGG
CAACGGCCATGACGAACTGACCGTTGCCGTGGTGGACATCGGCGCGACCATGACCACCCTCAGCGTGCTGCACCATGGTC
GAATCATCTATACCCGCGAGCAATTGTTCGGCGGTCGCCAGCTGACTGACGAAATTCAGCGCCGCTACGGGCTTTCCATG
GAAGAGGCCGGGCTGGCCAAGAAGCAGGGCGGGCTGCCTGACGATTATGTCAGCGAGGTGCTGGACCCCTTCAAGGACGC
GCTGGTGCAACAGGTTTCCCGATCCCTGCAGTTCTTCTTTGCGGCCGGTCAGTACAACTCCGTGGATCACATCATGCTGG
CAGGCGGTACGGCGTCGATTTCAGGGCTGGAGCATTTGATTCAGCGACGTATCGGCACACCGACCATGGTTGCCAACCCG
TTCGCCGACATGGCCTTGAGTTCCAAGGTCAATGCCGGTGCGCTGGCCAGCGATGCACCTGCCCTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A3M3ZC03

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.215	100	0.562
comM	Acinetobacter nosocomialis M2	56.215	100	0.562
comM	Acinetobacter baylyi ADP1	53.672	100	0.537
pilM	Legionella pneumophila strain ERS1305867	46.761	100	0.469