Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   JG741_RS08990 Genome accession   NZ_AP024210
Coordinates   1764536..1765036 (+) Length   166 a.a.
NCBI ID   WP_011677241.1    Uniprot ID   A0A0M2ZSZ2
Organism   Lactococcus cremoris strain G3-2     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1743802..1768911 1764536..1765036 within 0


Gene organization within MGE regions


Location: 1743802..1768911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JG741_RS08845 (LLG32_18260) - 1743802..1744002 (-) 201 WP_021164829.1 hypothetical protein -
  JG741_RS08850 (LLG32_18270) - 1744742..1745467 (-) 726 WP_126354613.1 hypothetical protein -
  JG741_RS08855 (LLG32_18280) - 1745460..1746782 (-) 1323 WP_011835935.1 AAA family ATPase -
  JG741_RS08860 (LLG32_18290) - 1747229..1747561 (-) 333 WP_011835936.1 hypothetical protein -
  JG741_RS08865 (LLG32_18300) - 1747561..1748001 (-) 441 WP_126354615.1 terminase small subunit -
  JG741_RS08870 (LLG32_18310) - 1748122..1748703 (-) 582 WP_126354617.1 hypothetical protein -
  JG741_RS08875 (LLG32_18320) - 1749166..1750839 (-) 1674 WP_126354619.1 hypothetical protein -
  JG741_RS08880 (LLG32_18330) - 1750948..1751277 (-) 330 WP_021212130.1 hypothetical protein -
  JG741_RS08885 (LLG32_18340) - 1751264..1751458 (-) 195 WP_126354621.1 hypothetical protein -
  JG741_RS14320 (LLG32_18350) - 1751455..1751709 (-) 255 WP_126354623.1 hypothetical protein -
  JG741_RS08890 (LLG32_18360) - 1751706..1751945 (-) 240 WP_126354624.1 hypothetical protein -
  JG741_RS08895 (LLG32_18370) - 1751982..1752263 (-) 282 WP_126354626.1 hypothetical protein -
  JG741_RS08900 (LLG32_18380) - 1752256..1752492 (-) 237 WP_126354628.1 hypothetical protein -
  JG741_RS08905 (LLG32_18390) - 1752489..1753010 (-) 522 WP_126354630.1 hypothetical protein -
  JG741_RS08910 (LLG32_18400) - 1753014..1753208 (-) 195 WP_032951169.1 DUF1655 domain-containing protein -
  JG741_RS08915 (LLG32_18410) - 1753327..1753512 (-) 186 WP_063282780.1 helix-turn-helix domain-containing protein -
  JG741_RS08920 (LLG32_18420) - 1753595..1753921 (+) 327 WP_063282781.1 hypothetical protein -
  JG741_RS08925 (LLG32_18430) - 1753997..1754245 (-) 249 WP_126354632.1 hypothetical protein -
  JG741_RS08930 (LLG32_18440) - 1754249..1754446 (-) 198 WP_014573247.1 hypothetical protein -
  JG741_RS08935 (LLG32_18450) - 1754706..1755092 (+) 387 WP_126354634.1 helix-turn-helix domain-containing protein -
  JG741_RS08940 (LLG32_18460) - 1755102..1755641 (+) 540 WP_126354636.1 hypothetical protein -
  JG741_RS08945 (LLG32_18470) - 1755962..1757146 (+) 1185 WP_126354638.1 tyrosine-type recombinase/integrase -
  JG741_RS08950 (LLG32_18480) - 1757436..1757885 (+) 450 WP_011677249.1 hypothetical protein -
  JG741_RS08955 (LLG32_18490) - 1757919..1758191 (-) 273 WP_011677248.1 chorismate mutase -
  JG741_RS08960 (LLG32_18500) - 1758275..1758763 (+) 489 WP_011677247.1 GAF domain-containing protein -
  JG741_RS08965 (LLG32_18510) - 1758795..1759436 (+) 642 WP_011677246.1 phosphotransferase -
  JG741_RS08970 (LLG32_18520) dnaX 1759482..1761143 (+) 1662 WP_011677245.1 DNA polymerase III subunit gamma/tau -
  JG741_RS08975 (LLG32_18530) - 1761177..1762643 (-) 1467 WP_021164830.1 amino acid permease -
  JG741_RS08980 (LLG32_18540) - 1763165..1764019 (+) 855 WP_021164831.1 undecaprenyl-diphosphate phosphatase -
  JG741_RS08985 (LLG32_18550) rpsF 1764213..1764506 (+) 294 WP_011677242.1 30S ribosomal protein S6 -
  JG741_RS08990 (LLG32_18560) ssb 1764536..1765036 (+) 501 WP_011677241.1 single-stranded DNA-binding protein Machinery gene
  JG741_RS08995 (LLG32_18570) rpsR 1765208..1765453 (+) 246 WP_003131952.1 30S ribosomal protein S18 -
  JG741_RS09000 (LLG32_18580) - 1765584..1766369 (+) 786 WP_021164832.1 metallophosphoesterase -
  JG741_RS09005 (LLG32_18590) - 1766442..1766750 (-) 309 WP_011836103.1 DUF960 domain-containing protein -
  JG741_RS09010 (LLG32_18600) - 1766789..1767637 (-) 849 WP_011677238.1 MurR/RpiR family transcriptional regulator -
  JG741_RS09015 (LLG32_18610) gnd 1768006..1768911 (+) 906 WP_011677237.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -

Sequence


Protein


Download         Length: 166 a.a.        Molecular weight: 18233.05 Da        Isoelectric Point: 5.2166

>NTDB_id=84089 JG741_RS08990 WP_011677241.1 1764536..1765036(+) (ssb) [Lactococcus cremoris strain G3-2]
MINNVVLVGRITRDPELRYTPQNQAVATFSLAVNRQFKNANGEREADFINCVIWRQQAENLANWAKKGALIGVTGRIQTR
NYENQQGQRVYVTEVVADSFQMLESRSARDGMGGGASAGSYSAPSQSTNNTPRPQTNNNSATPNFGRDADPFGSSPMEIS
DDDLPF

Nucleotide


Download         Length: 501 bp        

>NTDB_id=84089 JG741_RS08990 WP_011677241.1 1764536..1765036(+) (ssb) [Lactococcus cremoris strain G3-2]
ATGATTAACAATGTTGTATTAGTGGGACGCATTACTCGCGATCCTGAACTTCGTTACACCCCTCAAAATCAAGCTGTTGC
TACTTTTTCATTGGCTGTAAATCGTCAATTTAAAAATGCTAACGGTGAACGTGAGGCTGATTTCATTAACTGCGTTATTT
GGCGCCAACAAGCTGAAAATTTGGCAAATTGGGCTAAAAAAGGAGCTTTGATCGGTGTAACTGGTCGAATTCAAACACGT
AATTATGAAAATCAACAAGGTCAACGCGTTTATGTGACTGAGGTTGTGGCTGATAGTTTCCAAATGTTGGAAAGTAGATC
TGCTCGCGATGGTATGGGAGGCGGAGCTTCTGCCGGTTCATATTCTGCACCAAGCCAATCTACAAATAATACTCCACGTC
CACAAACGAATAATAATAGTGCAACACCGAATTTCGGTCGTGATGCTGACCCATTTGGTAGCTCACCTATGGAAATCTCG
GATGATGACCTTCCATTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A0M2ZSZ2

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

59.884

100

0.62

  ssbA Bacillus subtilis subsp. subtilis str. 168

56.395

100

0.584

  ssbB Bacillus subtilis subsp. subtilis str. 168

58.491

63.855

0.373

  ssb Glaesserella parasuis strain SC1401

33.898

100

0.361


Multiple sequence alignment