NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	QPR36_RS12010	Genome accession	NZ_CP126577
Coordinates	2509967..2510581 (-)	Length	204 a.a.
NCBI ID	WP_015387983.1	Uniprot ID	A0A6A8LHQ3
Organism	Bacillus velezensis strain 411
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2504967..2515581

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QPR36_RS11980 (QPR36_11980)	rpsT	2505270..2505536 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
QPR36_RS11985 (QPR36_11985)	holA	2505553..2506594 (-)	1042	Protein_2315	DNA polymerase III subunit delta	-
QPR36_RS11990 (QPR36_11990)	-	2506634..2506788 (-)	155	Protein_2316	hypothetical protein	-
QPR36_RS11995 (QPR36_11995)	-	2506829..2506963 (+)	135	WP_003152870.1	YqzM family protein	-
QPR36_RS12000 (QPR36_12000)	comEC	2506982..2509330 (-)	2349	WP_302239903.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
QPR36_RS12005 (QPR36_12005)	-	2509331..2509900 (-)	570	WP_003152868.1	ComE operon protein 2	-
QPR36_RS12010 (QPR36_12010)	comEA	2509967..2510581 (-)	615	WP_015387983.1	helix-hairpin-helix domain-containing protein	Machinery gene
QPR36_RS12015 (QPR36_12015)	comER	2510640..2511461 (+)	822	WP_160249252.1	late competence protein ComER	-
QPR36_RS12020 (QPR36_12020)	-	2511530..2512267 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
QPR36_RS12025 (QPR36_12025)	rsfS	2512264..2512620 (-)	357	WP_003152864.1	ribosome silencing factor	-
QPR36_RS12030 (QPR36_12030)	yqeK	2512617..2513198 (-)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
QPR36_RS12035 (QPR36_12035)	-	2513188..2513757 (-)	570	WP_302239909.1	nicotinate-nucleotide adenylyltransferase	-
QPR36_RS12040 (QPR36_12040)	yhbY	2513768..2514058 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
QPR36_RS12045 (QPR36_12045)	aroE	2514052..2514894 (-)	843	WP_302239913.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22255.41 Da Isoelectric Point: 5.5211

                                >NTDB_id=838886 QPR36_RS12010 WP_015387983.1 2509967..2510581(-) (comEA) [Bacillus velezensis strain 411]

MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=838886 QPR36_RS12010 WP_015387983.1 2509967..2510581(-) (comEA) [Bacillus velezensis strain 411]

ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG

CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A6A8LHQ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363

Detailed information NTG0838886