Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   U8327_RS08665 Genome accession   NZ_CP141765
Coordinates   1764532..1764999 (+) Length   155 a.a.
NCBI ID   WP_000338336.1    Uniprot ID   -
Organism   Escherichia coli strain HYM1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1759532..1769999
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U8327_RS08620 (U8327_08620) rplP 1759642..1760052 (-) 411 WP_000941212.1 50S ribosomal protein L16 -
  U8327_RS08625 (U8327_08625) rpsC 1760065..1760766 (-) 702 WP_000529945.1 30S ribosomal protein S3 -
  U8327_RS08630 (U8327_08630) rplV 1760784..1761116 (-) 333 WP_000447529.1 50S ribosomal protein L22 -
  U8327_RS08635 (U8327_08635) rpsS 1761131..1761409 (-) 279 WP_001138117.1 30S ribosomal protein S19 -
  U8327_RS08640 (U8327_08640) rplB 1761426..1762247 (-) 822 WP_000301864.1 50S ribosomal protein L2 -
  U8327_RS08645 (U8327_08645) rplW 1762265..1762567 (-) 303 WP_000617544.1 50S ribosomal protein L23 -
  U8327_RS08650 (U8327_08650) rplD 1762564..1763169 (-) 606 WP_000424395.1 50S ribosomal protein L4 -
  U8327_RS08655 (U8327_08655) rplC 1763180..1763809 (-) 630 WP_000579833.1 50S ribosomal protein L3 -
  U8327_RS08660 (U8327_08660) rpsJ 1763842..1764153 (-) 312 WP_001181004.1 30S ribosomal protein S10 -
  U8327_RS08665 (U8327_08665) pilD 1764532..1764999 (+) 468 WP_000338336.1 prepilin peptidase Machinery gene
  U8327_RS08670 (U8327_08670) bfr 1764996..1765472 (-) 477 WP_000675515.1 bacterioferritin -
  U8327_RS08675 (U8327_08675) bfd 1765545..1765739 (-) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  U8327_RS08680 (U8327_08680) tuf 1765922..1767106 (-) 1185 WP_000031783.1 elongation factor Tu -
  U8327_RS08685 (U8327_08685) fusA 1767177..1769291 (-) 2115 WP_000124703.1 elongation factor G -
  U8327_RS08690 (U8327_08690) rpsG 1769388..1769858 (-) 471 WP_001138043.1 30S ribosomal protein S7 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 17034.17 Da        Isoelectric Point: 8.1255

>NTDB_id=837545 U8327_RS08665 WP_000338336.1 1764532..1764999(+) (pilD) [Escherichia coli strain HYM1]
MDAALPFLILYACLSTLLFFWDAKHGLLPDRFTCPLLWSGLLFYQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=837545 U8327_RS08665 WP_000338336.1 1764532..1764999(+) (pilD) [Escherichia coli strain HYM1]
ATGGACGCTGCCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCAACCCTACTTTTTTTCTGGGATGCAAAGCATGGCTT
GTTACCTGACAGGTTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTATCAAGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGCCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACATGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTATTGGTTTGCTTATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGTCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGCTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.882

81.935

0.368